Marcelo Ferreira Marcondes Machado

• Abstract

  Due to the emergence of bacteria resistant to current antibiotics, the development of new antibiotics remains one of the biggest challenges of modern medicine. In Gram negative bacilli, the production of ²-lactamases is the most important contributing factor for the acquisition of resistance to ²-lactam antimicrobials. Inhibitors developed for these ²-lactamases act in conjunction with ²-…

  Development of beta-lactamase inhibitors of clinical interest, AP.R

• Abstract

  Due to the emergence of bacteria resistant to current antibiotics, the development of new antibiotics remains one of the highest challenges of modern medicine. The emergence of mechanisms of resistance to the currently most used antimicrobials indicates the importance to develop new therapeutic methods. Thiamine has its biosynthesis present only in prokaryotic organisms, since eukaryotes …

  Cloning, expression and purification of recombinant Thiamine Monophosphate Kinase (ThiL) from clinically relevant enterobacteria, BP.IC

• Abstract

  Fibroblast Growth Factor 2 (FGF-2 or bFGF) is a prototype derivative of the FGF family, which in turn is involved in tumor expansion and development. In the literature, there are several reports of its involvement with melanoma, due to its high expression by malignant cells, disease progression and poor prognosis. On the other hand, an anti-FGF-2 monoclonal antibody (mAb) named 3F12E7, de…

  Optimize a system for obtaining humanized Fibroblast Growth Factor 2 (FGF-2h) with high yield using E.coli, BP.IC

• Abstract

  Due to the emergence of bacteria resistant to current antibiotics, the development of new antibiotics remains one of the greatest challenges of modern medicine. In Gram negative bacilli, the production of ²-lactamase is the most important contributing factor for the acquisition of resistance to ²-lactam antimicrobials. The inhibitors developed for these ²-lactamases act in conjunction wit…

  Cloning, expression and purification of KPC-66 carbapenemase., BP.IC

(Only some records are available in English at this moment)

• Abstract

  The main goal of the present project is to study the hinge movement that the peptidase, timet oligopeptidase (TOP) may undergo upon substrate or inhibitor binding. Fluorescence techniques will be used to study this conformational change in great detail. TOP constructs will be prepared equipped with two donor-donor fluorescent probes (Trp residues) or donor-acceptor probes (5-F Trp - p-nit…

  Development and use of fluorescence techniques for the study of protein conformational changes, BE.EP.PD