Mariana Fioramonte

• Abstract

  Schizophrenia consists on a chronic psychiatric disorder reaching 1% of world population. It is a multifactorial disease resulting of environmental and genetic factors. The pathology molecular mechanisms are mostly unknown, therefore several methodologies such as GWAS, transcriptome and proteome has been used to study and better understand the molecular basis concerning schizophrenia.Our …

  Establishment of the hnRNPs role on schizophrenia through protein interaction network mapping, BP.PD

• Abstract

  The study of structures and conformations of proteins and protein complexes is extremely important in biochemistry, since most biochemical processes are governed by such macromolecules interconneted by extensive networks of interaction. Chemical cross-linking coupled to mass spectrometry (MS) is a technique that allows the characterization of structural proteins and protein complexes, esp…

  Structural Proteomics by Chemical Cross-linking Coupled to Mass Spectrometry: Development, Fundamental Studies and Applications., BP.DR
• Cross-linking coupled to mass spectrometry: FERM/Miosin complex interaction mapping, BP.MS

(Only some records are available in English at this moment)

• Abstract

  Chemical cross-linking coupled to mass spectrometry (XLMS) is a great tool to investigate protein-protein interaction. In this technique, in vitro complexes are reacted with cross-linkers and the linkage sites are latter reliably identified by mass spectrometry. Although this technique has been used successfully to map protein interactions in cases of stable, high affinity complexes, the …

  In vivo cross-linking: a more comprehensive way to determine protein-protein interactions, BE.EP.PD

• Abstract

  The chemical cross-linking coupled to mass spectrometry (MS) is one of the main techniques for protein and protein complex structural characterization when none of the high resolution techniques can be applied. Our laboratory has specialized and worked successfully on developing and applying this technique in protein and protein complexes in vitro. The next big step for this technique is …

  New frontiers in structural proteomics: advances in in vivo experiments using the genetically encoded unnatural amino acids, BE.EP.DR