Chemical cross-linking coupled to mass spectrometry (XLMS) is a great tool to investigate protein-protein interaction. In this technique, in vitro complexes are reacted with cross-linkers and the linkage sites are latter reliably identified by mass spectrometry. Although this technique has been used successfully to map protein interactions in cases of stable, high affinity complexes, the need to work with in vitro samples makes it difficult to identify transient/low affinity interactors. The group of Prof. James Bruce (Univ. Washington) is the leader and pioneering group in performing XLMS experiments in vivo. In such experiments, the right condition to the complex formation is obtained and the results from in vivo experiments better reflects the interaction network inside the cell. In this project, we intend to apply this methodology on chemoresistant cancer cells to assembly a quantitative interaction network of transient interactions. This methodology could be further applied on Mariana's project in Brazil.
News published in Agência FAPESP Newsletter about the scholarship: