In vivo cross-linking: a more comprehensive way to determine protein-protein interactions

• Grant number: 18/10220-1
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Effective date (Start): October 01, 2018
• Effective date (End): September 30, 2019
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Daniel Martins-de-Souza
• Grantee: Mariana Fioramonte
• Supervisor: James E. Bruce
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Research place: University of Washington, United States
• Associated to the scholarship: 16/18715-4 - Establishment of the hnRNPs role on schizophrenia through protein interaction network mapping, BP.PD

Abstract

Chemical cross-linking coupled to mass spectrometry (XLMS) is a great tool to investigate protein-protein interaction. In this technique, in vitro complexes are reacted with cross-linkers and the linkage sites are latter reliably identified by mass spectrometry. Although this technique has been used successfully to map protein interactions in cases of stable, high affinity complexes, the need to work with in vitro samples makes it difficult to identify transient/low affinity interactors. The group of Prof. James Bruce (Univ. Washington) is the leader and pioneering group in performing XLMS experiments in vivo. In such experiments, the right condition to the complex formation is obtained and the results from in vivo experiments better reflects the interaction network inside the cell. In this project, we intend to apply this methodology on chemoresistant cancer cells to assembly a quantitative interaction network of transient interactions. This methodology could be further applied on Mariana's project in Brazil.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

WIPPEL, HELISA H.; FIORAMONTE, MARIANA; CHAVEZ, JUAN D.; BRUCE, JAMES E.. Deciphering the architecture and interactome of hnRNP proteins and enigmRBPs. MOLECULAR OMICS, v. 17, n. 4, p. 503-516, AUG 1 2021. (18/10220-1)