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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hyperalgesic and edematogenic effects of Secapin-2, a peptide isolated from Africanized honeybee (Apis mellifera) venom

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Author(s):
Mourelle, D. [1] ; Brigatte, P. [1] ; Bringanti, L. D. B. [1] ; De Souza, B. M. [1] ; Arcuri, H. A. [1] ; Gomes, P. C. [1] ; Baptista-Saidemberg, N. B. [2] ; Ruggiero Neto, J. [3] ; Palma, M. S. [1]
Total Authors: 9
Affiliation:
[1] Sao Paulo State Univ, UNESP, Inst Biosci Rio Claro, CEIS Dept Biol, Rio Claro, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Anat Cell Biol & Physiol & Biophys, Campinas, SP - Brazil
[3] Sao Paulo State Univ, UNESP, Dept Phys IBILCE, Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Peptides; v. 59, p. 42-52, SEP 2014.
Web of Science Citations: 6
Abstract

Honeybee stings are a severe public health problem. Bee venom contains a series of active components, including enzymes, peptides, and biogenic amines. The local reactions observed after envenoming include a typical inflammatory response and pain. Honeybee venom contains some well-known polycationic peptides, such as Melittin, Apamin, MCD peptide, Cardiopep, and Tertiapin. Secapin in honeybee venom was described 38 years ago, yet almost nothing is known about its action. A novel, variant form of this peptide was isolated from the venom of Africanized honeybees (Apis mellifera). This novel peptide, named Secapin-2, is 25 amino acid residues long. Conformational analyses using circular dichroism and molecular dynamics simulations revealed a secondary structure rich in strands and turns, stabilized by an intramolecular disulfide bridge. Biological assays indicated that Secapin-2 did not induce hemolysis, mast cell degranulation or chemotactic activities. However, Secapin-2 caused potent dose-related hyperalgesic and edematogenic responses in experimental animals. To evaluate the roles of prostanoids and lipid mediators in the hyperalgesia and edema induced by this peptide, Indomethacin and Zileuton were used to inhibit the cyclooxygenase and lipoxygenase pathways, respectively. The results showed that Zileuton partially blocked the hyperalgesia induced by Secapin-2 and decreased the edematogenic response. In contrast, Indomethacin did not interfere with these phenomena. Zafirlukast, a leukotriene receptor antagonist, blocked the Secapin-2 induced hyperalgesia and edematogenic response. These results indicate that Secapin-2 induces inflammation and pain through the lipoxygenase pathway in both phenomena. (C) 2014 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 04/07942-2 - Bioprospetion of the Brazilian arthropods fauna searching for leader drugs for rational development of novel pharmaceuticals and pesticides
Grantee:Mario Sergio Palma
Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
FAPESP's process: 11/51684-1 - System biology as experimental strategy for discovery of novel natural products in the fauna of venomous arthropods from São Paulo State
Grantee:Mario Sergio Palma
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants