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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immunoproteome of Aspergillus fumigatus Using Sera of Patients with Invasive Aspergillosis

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Author(s):
Virginio, Emylli D. [1] ; Kubitschek-Barreira, Paula H. [1] ; Batista, Marjorie Vieira [2, 3, 4] ; Schirmer, Marcelo R. [5] ; Abdelhay, Eliana [5] ; Shikanai-Yasuda, Maria A. [2, 3] ; Lopes-Bezerra, Leila M. [1]
Total Authors: 7
Affiliation:
[1] Univ Rio de Janeiro State UERJ, Lab Cellular Mycol & Prote, Inst Biol, BR-20550013 Rio De Janeiro - Brazil
[2] Univ Sao Paulo, Clin Hosp, Lab Immunol LIM 48, BR-05403000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med, Dept Infect & Parasit Dis, BR-05403000 Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Med, Clin Infect Dis, Clin Hosp, BR-05403900 Sao Paulo - Brazil
[5] Natl Canc Inst, Ctr Bone Marrow Transplants, BR-20230130 Rio De Janeiro - Brazil
Total Affiliations: 5
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 15, n. 8, p. 14505-14530, AUG 2014.
Web of Science Citations: 11
Abstract

Invasive aspergillosis is a life-threatening lung or systemic infection caused by the opportunistic mold Aspergillus fumigatus. The disease affects mainly immunocompromised hosts, and patients with hematological malignances or who have been submitted to stem cell transplantation are at high risk. Despite the current use of Platelia (TM) Aspergillus as a diagnostic test, the early diagnosis of invasive aspergillosis remains a major challenge in improving the prognosis of the disease. In this study, we used an immunoproteomic approach to identify proteins that could be putative candidates for the early diagnosis of invasive aspergillosis. Antigenic proteins expressed in the first steps of A. fumigatus germination occurring in a human host were revealed using 2-D Western immunoblots with the serum of patients who had previously been classified as probable and proven for invasive aspergillosis. Forty antigenic proteins were identified using mass spectrometry (MS/MS). A BLAST analysis revealed that two of these proteins showed low homology with proteins of either the human host or etiological agents of other invasive fungal infections. To our knowledge, this is the first report describing specific antigenic proteins of A. fumigatus germlings that are recognized by sera of patients with confirmed invasive aspergillosis who were from two separate hospital units. (AU)

FAPESP's process: 12/50212-1 - Aspergillus infections in immunocompromised patients: clinical-laboratory-molecular diagnosis and studies genotypic and phenotypic of isolates
Grantee:Maria Aparecida Shikanai Yasuda
Support Opportunities: Regular Research Grants