| Full text | |
| Author(s): |
Total Authors: 3
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| Affiliation: | [1] Univ Sao Paulo, Inst Biociencias, Dept Bot, BR-05508090 Sao Paulo - Brazil
Total Affiliations: 1
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| Document type: | Journal article |
| Source: | APPLICATIONS IN PLANT SCIENCES; v. 1, n. 9 SEP 2013. |
| Web of Science Citations: | 9 |
| Abstract | |
Premise of the study: New primers were developed for Bignonieae to enable phylogenetic studies within this clade using herbarium samples. Methods and Results: Internal primers were designed based on available sequences of the plastid ndhF gene and the rpl32-trnL intergenic spacer region, and the nuclear gene PepC. The resulting primers were used to amplify DNA extracted from herbarium materials. High-quality data were obtained from herbarium samples up to 53 yr old. Conclusions: The standardized methodology allows the inclusion of herbarium materials as alternative sources of DNA for phylogenetic studies in Bignonieae. (AU) | |
| FAPESP's process: | 12/50260-6 - Structure and evolution of the Amazonian biota and its environment: an integrative approach |
| Grantee: | Lúcia Garcez Lohmann |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 09/12508-3 - Molecular phylogeny of the genus Dolichandra s.l. |
| Grantee: | Luiz Henrique Martins Fonseca |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 11/50859-2 - Systematics of the tribe Bignonieae (Bignoniaceae) |
| Grantee: | Lúcia Garcez Lohmann |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 11/09160-5 - Taxonomic revision, phylogeny and biogeography of Bignonia L. (Bignonieae, Bignoniaceae) |
| Grantee: | Alexandre Rizzo Zuntini |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |