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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

MICROSATELLITE LOCI FOR ORTHOPHYTUM OPHIUROIDES (BROMELIOIDEAE, BROMELIACEAE) SPECIES ADAPTED TO NEOTROPICAL ROCK OUTCROPS

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Author(s):
Aoki-Goncalves, Felipe [1] ; Louzada, Rafael B. [2, 3] ; De Souza, Livia Moura ; Palma-Silva, Clarisse [4, 1]
Total Authors: 4
Affiliation:
[1] Inst Bot, BR-04301902 Sao Paulo - Brazil
[2] Univ Fed Pernambuco, Dept Bot, BR-50670901 Recife, PE - Brazil
[3] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP - Brazil
[4] Univ Estadual Paulista, Dept Ecol, BR-13506900 Rio Claro, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: APPLICATIONS IN PLANT SCIENCES; v. 2, n. 3 MAR 2014.
Web of Science Citations: 6
Abstract

Premise of the study: Microsatellite primers were developed for Orthophytum ophiuroides, a rupicolous bromeliad species endemic to neotropical rocky fields. These microsatellite loci will be used to investigate population differentiation and species cohesion in such fragmented environments. The loci were tested for cross-amplification in related bromeliad species. Methods and Results: Eleven polymorphic microsatellite markers were isolated and characterized from an enriched library of O. ophiuroides. The loci were tested on 42 individuals from two populations of this species. The number of alleles per locus ranged from three to nine and the expected and observed heterozygosities ranged from 0.167 to 0.870 and from 0.369 to 0.958, respectively. Seven loci successfully amplified in other related bromeliad species. Conclusions: Our results suggest that the microsatellite loci developed here will be useful to assess genetic diversity and gene flow in O. ophiuroides for the investigation of population differentiation and species cohesion in neotropical mountainous habitats. (AU)

FAPESP's process: 09/52725-3 - Speciation, reproductive isolation, and population genetics in species of Bromeliaceae family: implications for taxonomy, evolution and conservation
Grantee:Clarisse Palma da Silva
Support type: BIOTA-FAPESP Program - Young Investigators Grants
FAPESP's process: 09/17411-8 - Speciation, reproductive isolation, and population genetics in species of Bromeliaceae family: implications for taxonomy, evolution and conservation
Grantee:Clarisse Palma da Silva
Support type: Scholarships in Brazil - BIOTA - Young Researchers