An improved purification procedure for Leishmania RNA virus (LRV)

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Author(s):	de Souza, Marcos Michel ^[1] ; Manzine, Livia Regina ^[1] ; da Silva, Marcos Vinicius G. ^[2] ; Bettini, Jefferson ^[3] ; Portugal, Rodrigo Vilares ^[3] ; Cruz, Angela Kaysel ^[2] ; Arruda, Eurico ^[2] ; Thiemann, Otavio Henrique ^[1] Total Authors: 8
Affiliation:	^[1] Univ Sao Paulo, Inst Fis Sao Carlos, Dept Fis & Informat, Sao Carlos, SP - Brazil ^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, BR-14049 Ribeirao Preto, SP - Brazil ^[3] Ctr Nacl Pesquisa Energia & Mat, Lab Nacl Nanotecnol, Campinas, SP - Brazil Total Affiliations: 3
Document type:	Journal article
Source:	Brazilian Journal of Microbiology; v. 45, n. 2, p. 695-698, APR-JUN 2014.
Web of Science Citations:	1
Abstract
Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations. (AU)

FAPESP's process:	98/14138-2 - Center for Structural Molecular Biotechnology
Grantee:	Glaucius Oliva
Support Opportunities:	Research Grants - Research, Innovation and Dissemination Centers - RIDC