Immunoexpression of S100A4 in canine skin melanomas and correlation with histopathological parameters

Author(s)

Grandi, Fabrizio ^{[1, 2]} ; Rocha, Rafael M. ^[3] ; Miot, Helio A. ^[4] ; Cogliati, Bruno ^[5] ; Rocha, Noeme S. ^{[1, 2]}

Affiliation

^[1] Univ Estadual Paulista UNESP, Dept Vet Clin, Lab Invest & Comparat Pathol, Sch Vet Med & Anim Sci, Botucatu, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Dept Pathol, Botucatu Med Sch, BR-18618970 Botucatu, SP - Brazil

^[3] Fundacao Antonio Prudente, Natl Inst Oncogen, Hosp AC Camargo, Dept Oncol, BR-01509010 Sao Paulo - Brazil

^[4] Univ Estadual Paulista, UNESP, Botucatu Med Sch, Dept Dermatol, BR-18618970 Botucatu, SP - Brazil

^[5] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, BR-05508270 Sao Paulo - Brazil

• Document type: Journal article
• Source: VETERINARY QUARTERLY; v. 34, n. 2, p. 98-104, 2014.
• Web of Science Citations: 3

Abstract

Background: Melanoma is one of the most common skin neoplasms in humans and dogs. The tumor microenvironment in melanoma comprises cancer cells and stromal cells that interact to accelerate tumor progression. Several prognostic markers for melanomas have been studied in many human tumors, including fibroblast-specific protein 1 (S100A4). S100A4 is a member of the S100 family of calcium-binding proteins in stromal cells. Hypothesis/objectives: The objective of this study was to describe the immunohistochemical patterns of S100A4 in stroma and neoplastic cells of canine skin melanomas and correlate them with some histological parameters. Animals and Methods: Forty-eight samples (38 pigmented and 10 non-pigmented melanomas) were first selected and their nature confirmed using S100, Melan A and vimentin. All cases were examined by immunohistochemistry using S100A4 to correlate expression, histotype, and level of invasion. Results: All the tumors, including 10 non-pigmented, were positive for S100, Melan A, vimentin and negative for cytokeratin AE1/AE3 (consistent with melanomas). The 48 melanomas were classified as epithelioid (n = 21), spindle (n = 14), and mixed (n = 13). S100A4 was preferentially expressed in epithelioid and spindle cell types compared with mixed melanomas and S100A4 expression was not associated with level of invasion (Clark's levels IV to V). Conclusion: S100A4 expression in melanoma samples varied among histotypes but not between levels of invasion. (AU)

• FAPESP's process: 10/14317-8 - Cancer-associated fibroblasts and correlation with pathological parameters in canine cutaneous melanomas
• Grantee: Fabrizio Grandi
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 11/01610-1 - Evaluation of CD31, S100A4, and alpha SMA in the tumor microenvironment and their relation to endotelial-mesenchymal transition in canine spontaneous cutaneous melanomas
• Grantee: Noeme Sousa Rocha
• Support Opportunities: Regular Research Grants