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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Side-Effects of Thiamethoxam on the Brain and Midgut of the Africanized Honeybee Apis mellifera (Hymenopptera: Apidae)

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Author(s):
Oliveira, Regiane Alves [1] ; Roat, Thaisa Cristina [1] ; Carvalho, Stephan Malfitano [1] ; Malaspina, Osmar [1]
Total Authors: 4
Affiliation:
[1] UNESP Univ Estadual Paulista, Ctr Estudos Insetos Sociais, Dept Biol, Inst Biociencias Rio Claro, BR-13500900 Bela Vista, Rio Claro Sao P - Brazil
Total Affiliations: 1
Document type: Journal article
Source: ENVIRONMENTAL TOXICOLOGY; v. 29, n. 10, p. 1122-1133, OCT 2014.
Web of Science Citations: 40
Abstract

The development of agricultural activities coincides with the increased use of pesticides to control pests, which can also be harmful to nontarget insects such as bees. Thus, the goal of this work was assess the toxic effects of thiamethoxam on newly emerged worker bees of Apis mellifera (africanized honeybee-AHB). Initially, we determined that the lethal concentration 50 (LC50) of thiamethoxam was 4.28 ng a.i./mu L of diet. To determine the lethal time 50 (LT50), a survival assay was conducted using diets containing sublethal doses of thiamethoxam equal to 1/10 and 1/100 of the LC50. The group of bees exposed to 1/10 of the LC50 had a 41.2% reduction of lifespan. When AHB samples were analyzed by morphological technique we found the presence of condensed cells in the mushroom bodies and optical lobes in exposed honeybees. Through Xylidine Ponceau technique, we found cells which stained more intensely in groups exposed to thiamethoxam. The digestive and regenerative cells of the midgut from exposed bees also showed morphological and histochemical alterations, like cytoplasm vacuolization, increased apocrine secretion and increased cell elimination. Thus, intoxication with a sublethal doses of thiamethoxam can cause impairment in the brain and midgut of AHB and contribute to the honeybee lifespan reduction. (C) 2013 Wiley Periodicals, Inc. (AU)

FAPESP's process: 10/15628-7 - Morphological and histochemical analysis of the midgut and brain from newly-emerged workers of Apis mellifera L. treated with Thiamethoxam
Grantee:Regiane Alves de Oliveira
Support Opportunities: Scholarships in Brazil - Scientific Initiation