Alteration of sFAS and sFAS ligand expression during canine visceral leishmaniosis

Author(s)

Perosso, Juliana ^[1] ; Oliveira Silva, Kathlenn Liezbeth ^[1] ; de Souza Ferreira, Stefani Iris ^[1] ; Avanco, Saulo Vinicius ^[1] ; Patto dos Santos, Paulo Sergio ^[1] ; Eugenio, Flavia de Rezende ^[1] ; Martins de Almeida, Breno Fernando ^[1] ; Felix de Lima, Valeria Marcal ^[1]

Affiliation

^[1] Sao Paulo State Univ, Dept Clin Care Surg & Anim Reprod, Cellular Immunol Lab, FMVA, UNESP, Sch Vet Sci, Fac Med Vet, Univ Estadual Julio de Me, BR-16050680 Sao Paulo - Brazil

• Document type: Journal article
• Source: Veterinary Parasitology; v. 205, n. 3-4, p. 417-423, OCT 15 2014.
• Web of Science Citations: 12

Abstract

Visceral leishmaniosis (VL) is caused by intracellular parasites of the genus Leishmania that affect humans and several animal species. Dogs are one of the main urban reservoirs of Leishmania infantum and play a central role in the transmission cycle to humans via sandflies. CD3+ cells apoptosis is involved in the immune response in VL. Dysregulation of apoptosis has been implicated in various disease states. An important regulator of apoptosis is the FAS-FAS-associated death domain protein (cluster of differentiation 95 - CD95) and FASL-FAS ligand protein (cluster of differentiation 178 - CD178) system involved in the down-regulation of immune reactions and in T cell-mediated cytotoxicity. FAS is a member of the tumor necrosis factor (TNF) receptor super family, which can be expressed in transmembrane or soluble forms. The soluble levels of FAS (sFAS), FASL (sFASL) and active Caspase-3, this last related to apoptotic cascade, were investigated in the spleen of 19 symptomatic dogs presenting moderate VL and 6 healthy dogs, determined by ELISA assay. The splenic parasite load was determined by real-time PCR monitoring of amplification of the intergenic internal transcribed spacer (ITS1) gene of parasite rRNA. sFAS levels were lower (p < 0.05). sFASL and active Caspase-3 levels were higher (p < 0.05)in dogs with VL compared with controls. Negative correlation was observed between parasite burden and sFASL levels. The increase in sFASL could be related to the mechanism involved in the elimination of the parasite. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 11/06214-7 - Role of Fas and TNF in the apoptosis of CD4 T lymphocyte and CD8 T lymphocyte in dogs with visceral leishmaniasis
• Grantee: Valéria Marçal Felix de Lima
• Support type: Regular Research Grants