Toll like receptors gene expression of human keratinocytes cultured of severe burn injury

Author(s)

Mac Cornick, Sarita ^[1] ; Alves Correa de Noronha, Silvana Aparecida ^[1] ; Ribeiro de Noronha, Samuel Marcos ^[1] ; Cezillo, Marcus V. B. ^[1] ; Ferreira, Lydia Masako ^[1] ; Gragnani, Alfredo ^[1]

Affiliation

^[1] Fed Univ Sao Paulo UNIFESP, Paulista Sch Med EPM, Dept Surg, Div Plast Surg, Sao Paulo - Brazil

• Document type: Journal article
• Source: Acta Cirurgica Brasileira; v. 29, n. 3, p. 33-38, 2014.
• Web of Science Citations: 2

Abstract

PURPOSE: To evaluate the expression profile of genes related to Toll Like Receptors (TLR) pathways of human Primary Epidermal keratinocytes of patients with severe burns. METHODS: After obtaining viable fragments of skin with and without burning, culture hKEP was initiated by the enzymatic method using Dispase (Sigma-Aldrich). These cells were treated with Trizol(R) (Life Technologies) for extraction of total RNA. This was quantified and analyzed for purity for obtaining cDNA for the analysis of gene expression using specific TLR pathways PCR Arrays plates (SA Biosciences). RESULTS: After the analysis of gene expression we found that 21% of these genes were differentially expressed, of which 100% were repressed or hyporegulated. Among these, the following genes (fold decrease): HSPA1A (-58), HRAS (-36), MAP2K3 (-23), TOLLIP (-23), RELA (-18), FOS (-16), and TLR1 (-6.0). CONCLUSIONS: This study contributes to the understanding of the molecular mechanisms related to TLR pathways and underlying wound infection caused by the burn. Furthermore, it may provide new strategies to restore normal expression of these genes and thereby change the healing process and improve clinical outcome. (AU)

• FAPESP's process: 11/12945-4 - Keratinocyte growth factor, interleukin 1 beta, 6, 8, 10, 12 and tumor necrosis factor alpha in burned patients
• Grantee: Alfredo Gragnani Filho
• Support Opportunities: Regular Research Grants