Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antiplatelet drugs reduce the immunoinflammatory response in a rat model of periodontal disease

Full text
Author(s):
Coimbra, L. S. [1] ; Steffens, J. P. [1] ; Muscara, M. N. [2] ; Rossa, Jr., C. [3] ; Spolidorio, L. C. [1]
Total Authors: 5
Affiliation:
[1] UNESP Univ Estadual Paulista, Dept Physiol & Pathol, Fac Odontol Araraquara, Araraquara, SP - Brazil
[2] Univ Sao Paulo, Dept Pharmacol, Inst Biomed Sci, Sao Paulo - Brazil
[3] UNESP Univ Estadual Paulista, Dept Diag & Surg, Fac Odontol Araraquara, Araraquara, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF PERIODONTAL RESEARCH; v. 49, n. 6, p. 729-735, DEC 2014.
Web of Science Citations: 3
Abstract

Background and ObjectiveAfter activation, platelets express mediators that modulate inflammation. We hypothesized that drug-induced platelet inactivation may interfere in the inflammatory process in experimental periodontal disease by suppressing the release of biological mediators to the injury site. Material and MethodsTo evaluate the effects of antiplatelet drugs on experimental periodontal disease, 60 rats were randomly assigned to six groups (n=10) and ligatures were placed around lower first molars in three groups. The other three groups were not subjected to the induction of periodontal disease and were used as negative controls. During the experimental period, animals were given aspirin (30mg/kg) or clopidogrel (75mg/kg) intragastrically once daily for 3d. On day 3, they were killed and gingival tissue were used to evaluate myeloperoxidase activity and the expression of the chemokine CXCL4. Hemi-mandibles were used for microscopic evaluation. ResultsClopidogrel significantly reduced the inflammatory infiltrate and increased the amount of collagen fibers. Histometric analysis showed that clopidogrel impaired alveolar bone loss. Expression of CXCL4 was significantly increased (p<0.001) in rats subjected to periodontal disease. Systemic administration of aspirin and clopidogrel induced a significant decrease ( p<0.05) in the expression of CXCL4. Treatment with antiplatelet drugs resulted in a significant reduction of myeloperoxidase activity when compared to saline-treated animals with periodontal disease. ConclusionClopidogrel but not aspirin showed the ability of preventing bone loss in experimental periodontitis. (AU)

FAPESP's process: 10/10715-9 - Evaluation of cytokines elaborated for platelet during the development of periodontal repair and disease in rats treated or not with antiplatelets drugs
Grantee:Luis Carlos Spolidorio
Support Opportunities: Regular Research Grants