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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Influence of post-starvation extraction time and prey-specific diet in Tityus serrulatus scorpion venom composition and hyaluronidase activity

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Author(s):
Pucca, Manuela Berto [1] ; Amorim, Fernanda Gobbi [1] ; Cerni, Felipe Atigusto [1] ; Figueiredo Bordon, Karla de Castro [1] ; Cardoso, Iara Aime [1] ; Pino Anjolette, Fernando Antonio [1] ; Arantes, Eliane Candiani [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Chem & Phys, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Toxicon; v. 90, p. 326-336, NOV 2014.
Web of Science Citations: 19
Abstract

The role of diet in venom composition has been a topic of intense research interest. This work presents evidence that the variation in the venom composition from the scorpion Tityus serrulatus (Ts) is closely associated with post-starvation extraction time and prey-specific diet. The scorpions were fed with cockroach, cricket, peanut beetle or giant Tenebrio. The venoms demonstrated a pronounced difference in the total protein and toxins composition, which was evaluated by electrophoresis, reversed-phase chromatography, densitometry, hyaluronidase activity and N-terminal sequencing. Indeed, many toxins and peptides, such as Ts1, Ts2, Ts4, Ts5, Ts6, Ts15, Ts19 frag. II, hypotensins 1 and 3, PAPE peptide and peptide 9797 (first described in Ts venom), were all identified in different proportions in the analyzed Ts venoms. This study is pioneer on assessing the influence of the starvation time and the prey diet on hyaluronidase activity as well as to describe a modification of Tricine-gel-electrophoresis to evaluate this enzyme activity. Altogether, this study reveal a large contribution of the extraction time and diet on Ts venom variability as well as present a background to recommend the cockroach diet to obtain higher protein content and the cricket diet to obtain higher hyaluronidase specific activity. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 12/12954-6 - Study of the toxins Ts6 e Ts15 from the scorpion Tityus serrulatus as potential therapeutic drugs for the treatment of autoimmune diseases
Grantee:Manuela Berto Pucca
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 11/12317-3 - Cloning and heterologous expression of hyaluronidase and/or novel toxins obtained from the transcriptome of Tityus serrulatus' venom gland
Grantee:Fernanda Gobbi Amorim
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/13590-8 - Isolation, molecular and functional characterization of a new toxin from Tityus serrulatus scorpion venom
Grantee:Felipe Augusto Cerni
Support Opportunities: Scholarships in Brazil - Doctorate