Tityus serrulatus scorpion venom effects on inflammatory mediators production by ...
| Full text | |
| Author(s): |
Pucca, Manuela Berto
[1]
;
Amorim, Fernanda Gobbi
[1]
;
Cerni, Felipe Atigusto
[1]
;
Figueiredo Bordon, Karla de Castro
[1]
;
Cardoso, Iara Aime
[1]
;
Pino Anjolette, Fernando Antonio
[1]
;
Arantes, Eliane Candiani
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Chem & Phys, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Toxicon; v. 90, p. 326-336, NOV 2014. |
| Web of Science Citations: | 19 |
| Abstract | |
The role of diet in venom composition has been a topic of intense research interest. This work presents evidence that the variation in the venom composition from the scorpion Tityus serrulatus (Ts) is closely associated with post-starvation extraction time and prey-specific diet. The scorpions were fed with cockroach, cricket, peanut beetle or giant Tenebrio. The venoms demonstrated a pronounced difference in the total protein and toxins composition, which was evaluated by electrophoresis, reversed-phase chromatography, densitometry, hyaluronidase activity and N-terminal sequencing. Indeed, many toxins and peptides, such as Ts1, Ts2, Ts4, Ts5, Ts6, Ts15, Ts19 frag. II, hypotensins 1 and 3, PAPE peptide and peptide 9797 (first described in Ts venom), were all identified in different proportions in the analyzed Ts venoms. This study is pioneer on assessing the influence of the starvation time and the prey diet on hyaluronidase activity as well as to describe a modification of Tricine-gel-electrophoresis to evaluate this enzyme activity. Altogether, this study reveal a large contribution of the extraction time and diet on Ts venom variability as well as present a background to recommend the cockroach diet to obtain higher protein content and the cricket diet to obtain higher hyaluronidase specific activity. (C) 2014 Elsevier Ltd. All rights reserved. (AU) | |
| FAPESP's process: | 11/12317-3 - Cloning and heterologous expression of hyaluronidase and/or novel toxins obtained from the transcriptome of Tityus serrulatus' venom gland |
| Grantee: | Fernanda Gobbi Amorim |
| Support type: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 12/12954-6 - Study of the toxins Ts6 e Ts15 from the scorpion Tityus serrulatus as potential therapeutic drugs for the treatment of autoimmune diseases |
| Grantee: | Manuela Berto Pucca |
| Support type: | Scholarships in Brazil - Post-Doctorate |
| FAPESP's process: | 12/13590-8 - Isolation, molecular and functional characterization of a new toxin from Tityus serrulatus scorpion venom |
| Grantee: | Felipe Augusto Cerni |
| Support type: | Scholarships in Brazil - Doctorate |