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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development and characterization of a cationic lipid nanocarrier as non-viral vector for gene therapy

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Author(s):
Severino, Patricia [1, 2] ; Szymanski, Marcelo [3] ; Favaro, Marianna [3] ; Azzoni, Adriano R. [3] ; Chaud, Marco V. [4] ; Santana, Maria Helena A. [1] ; Silva, Amelia M. [5, 6] ; Souto, Eliana B. [2, 7]
Total Authors: 8
Affiliation:
[1] Univ Campinas Unicamp, Sch Chem Engn, Dept Biotechnol Proc, BR-13083970 Campinas, SP - Brazil
[2] Fernando Pessoa Univ UFP FCS, Fac Hlth Sci, P-4200150 Oporto - Portugal
[3] Univ Estadual Campinas, Mol Biol & Genet Engn Ctr, Lab Genet & Mol Anal, Campinas, SP - Brazil
[4] Sorocaba Univ, UNISO, Lab Dev & Evaluat Bioact Substance, Sorocaba, SP - Brazil
[5] Univ Tras Os Montes & Alto Douro UTAD, Dept Biol & Environm, P-5001801 Vila Real - Portugal
[6] Ctr Res & Technol Agroenvironm & Biol Sci CITAB U, P-5001801 Vila Real - Portugal
[7] Univ Coimbra FFUC, Fac Pharm, P-3000548 Coimbra - Portugal
Total Affiliations: 7
Document type: Journal article
Source: European Journal of Pharmaceutical Sciences; v. 66, p. 78-82, JAN 23 2015.
Web of Science Citations: 25
Abstract

The aim of the present work was to produce a cationic solid lipid nanoparticle (SLN) as non-viral vector for protein delivery. Cationic SLN were produced by double emulsion method, composed of softisan (R) 100, cetyltrimethylammonium bromide (CTAB), Tween (R) 80, Span (R) 80, glycerol and lipoid (R) S75 loading insulin as model protein. The formulation was characterized in terms of mean hydrodynamic diameter (z-ave), polydispersity index (PI), zeta potential (ZP), stability during storage time, stability after lyophilization, effect of toxicity and transfection ability in HeLa cells, in vitro release profile and morphology. SLN were stable for 30 days and showed minimal changes in their physicochemical properties after lyophilization. The particles exhibited a relatively slow release, spherical morphology and were able to transfect HeLa cells, but toxicity remained an obstacle. Results suggest that SLN are nevertheless promising for delivery of proteins or nucleic acids for gene therapy. (C) 2014 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 08/00421-8 - Incorporation and immunological evaluation of tumor antigens incorporated in solid lipid nanoparticles and solid lipid nanocarriers
Grantee:Patricia Severino
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 11/20801-2 - Polymixin B load in solid lipid nanoparticles for incorporation in mucoadhesive hydrogels for oral mucositis treatment
Grantee:Patricia Severino
Support Opportunities: Scholarships in Brazil - Post-Doctoral