Polymixin B load in solid lipid nanoparticles for incorporation in mucoadhesive hydrogels for oral mucositis treatment

• Grant number: 11/20801-2
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): November 01, 2012
• Effective date (End): October 31, 2014
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Maria Helena Andrade Santana
• Grantee: Patricia Severino
• Host Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The dispersions of solid lipid nanoparticles (SLN) are currently being studied for various therapy applications, to overcome problems of physical and chemical stability, cost and encapsulation efficiency associated with conventional liposomes. Oral mucositis is a comorbid affecting patients in treatment of head and neck cancer. Conventional therapies are palliative, such as local anesthetic and cryosurgery and/or involves, also, the use of local anti-inflammatory and antibiotics which action efficiency is reduced by the low residence time at the site of action. The local application of laser is restricted due to higher cost. The main purpose of the project is developing a multifunctional pharmaceutical form with low cost for topical oral use with anesthetic, antimicrobial and healing effects for the treatment of oral mucositis. This pharmaceutical form is composed of a polymer film formed by electrostatic binding of polyelectrolytes, chitosan (antimicrobial) and hyaluronic acid (healing), incorporating lidocaine hydrochloride (anesthetic). Additionally, in this film will be incorporated SLN encapsulating the antibiotic polymyxin B sulfate to increase the efficiency of action by controlled release and increased residence time at the site of action. Results of this study will improve the quality of life of patients undergoing radiotherapy and chemotherapy for cancer (by reducing the pain, that strongly, affects their ability to supply), to prevent the risk of comorbidity (because of weak immune deficiency), and reduce the time between applications of cancer treatment (for better conditions offered to patients). In parallel, the study also opens up new possibilities for the treatment of mucositis as the other of the pharynx and vaginal cavity.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS, RAFAEL SILVA; LOUREIRO, KAHYNNA CAVALCANTE; REZENDE, POLYANA SANTOS; ANDRADE, LUCIANA NALONE; BARBOSA, RAQUEL DE MELO; SANTINI, ANTONELLO; SANTOS, ANA CLAUDIA; DA SILVA, CLASSIUS FERREIRA; SOUTO, ELIANA BARBOSA; DE SOUSA, DAMIAO PERGENTINO; et al. Innovative nanocompounds for cutaneous administration of classical antifungal drugs: a systematic review. JOURNAL OF DERMATOLOGICAL TREATMENT, v. 30, n. 6, APR 2019. (08/00421-8, 11/20801-2)

SEVERINO, PATRICIA; CHAUD, MARCO V.; SHIMOJO, ANDREA; ANTONINI, DANILO; LANCELLOTI, MARCELO; SANTANA, MARIA HELENA A.; SOUTO, ELIANA B.. Sodium alginate-cross-linked polymyxin B sulphate-loaded solid lipid nanoparticles: Antibiotic resistance tests and HaCat and NIH/3T3 cell viability studies. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 129, p. 191-197, MAY 1 2015. (11/20801-2)

BARBOSA, R. M.; SEVERINO, P.; PRETE, P. S. C.; SANTANA, M. H. A.. Influence of different surfactants on the physicochemical properties of elastic liposomes. PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY, v. 22, n. 3, p. 360-369, 2017. (02/12637-9, 11/20801-2)

SEVERINO, P.; SILVEIRA, E. F.; VAZZANA, M.; CHAUD, M. V.; SANTANA, M. H. A.; SOUTO, E. B.. Validation of an UV spectrophotometric assay for the quantification of polymyxin B in solid lipid nanoparticles. Pharmazie, v. 70, n. 11, p. 693-697, NOV 2015. (11/20801-2)

SEVERINO, PATRICIA; SZYMANSKI, MARCELO; FAVARO, MARIANNA; AZZONI, ADRIANO R.; CHAUD, MARCO V.; SANTANA, MARIA HELENA A.; SILVA, AMELIA M.; SOUTO, ELIANA B.. Development and characterization of a cationic lipid nanocarrier as non-viral vector for gene therapy. European Journal of Pharmaceutical Sciences, v. 66, p. 78-82, JAN 23 2015. (08/00421-8, 11/20801-2)

DA SILVA, CLASSIUS FERREIRA; SEVERINO, PATRICIA; MARTINS, FERNANDA; SANTANA, MARIA HELENA A.; SOUTO, ELIANA B.. Didanosine-loaded chitosan microspheres optimized by surface-response methodology: A modified ``Maximum Likelihood Classification'' approach formulation for reverse transcriptase inhibitors. BIOMEDICINE & PHARMACOTHERAPY, v. 70, p. 46-52, MAR 2015. (08/00421-8, 11/20801-2)