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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Angiotensin II restricted analogs with biological activity in the erythrocytic cycle of Plasmodium falciparum

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Author(s):
Torossian Torres, Marcelo Der [1] ; Silva, Adriana Farias [1] ; Silva, Leandro de Souza [2] ; de Sa Pinheiro, Ana Acacia [2] ; Jr Oliveira, Vani Xavier [3]
Total Authors: 5
Affiliation:
[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Santo Andre, SP - Brazil
[2] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas, Rio De Janeiro, RJ - Brazil
[3] Jr Oliveira, Jr., Vani Xavier, Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Santo Andre, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF PEPTIDE SCIENCE; v. 21, n. 1, p. 24-28, JAN 2015.
Web of Science Citations: 6
Abstract

The anti-plasmodial activity of conformationally restricted analogs of angiotensin II against Plasmodium gallinaceum has been described. To observe activity against another Plasmodium species, invasion of red blood cells by Plasmodium falciparum was analyzed. Analogs restricted with lactam or disulfide bridges were synthesized to determine their effects and constraints in the peptide-parasite interaction. The analogs were synthesized using tert-butoxycarbonyl and fluoromethoxycarbonyl solid phase methods, purified by liquid chromatography, and characterized by mass spectrometry. Results indicated that the lactam bridge restricted analogs 1 (Glu-Asp-Arg-Orn-Val-Tyr-Ile-His-Pro-Phe) and 3 (Asp-Glu-Arg-Val-Orn-Tyr-Ile-His-Pro-Phe) showed activity toward inhibition of ring formation stage of P. falciparum erythrocytic cycle, preventing invasion in about 40% of the erythrocytes. The disulfide-bridged analog 10 (Cys-Asp-Arg-Cys-Val-Tyr-Ile-His-Pro-Phe) was less effective yet significant, showing a 25% decrease in infection of new erythrocytes. In all cases, the peptides presented no pressor activity, and hydrophobic interactions between the aromatic and alkyl amino acid side chains were preserved, a factor proven important in efficacy against P. gallinaceum. In contrast, hydrophilic interactions between the Asp1 carboxyl and Arg2 guanidyl groups proved not to be as important as they were in the case of P. gallinaceum, while interactions between the Arg2 guanidyl and Tyr4 hydroxyl groups were not important in either case. The beta-turn conformation was predominant in all of the active peptides, proving importance in anti-plasmodial activity. This approach provides insight for understanding the importance of each amino acid residue on the native angiotensin II structure and a new direction for the design of potential chemotherapeutic agents. Copyright (C) 2014 European Peptide Society and John Wiley \& Sons, Ltd. (AU)

FAPESP's process: 11/15083-3 - Cyclic Antimalariais Derivates of Angiotensin II
Grantee:Marcelo Der Torossian Torres
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 11/10823-9 - Antimalarial compounds derivative from angiotensin II
Grantee:Vani Xavier de Oliveira Junior
Support Opportunities: Regular Research Grants