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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Therapeutic Administration of Recombinant Paracoccin Confers Protection against Paracoccidioides brasiliensis Infection: Involvement of TLRs

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Author(s):
Paiva Alegre-Maller, Ana Claudia [1] ; Mendonca, Flavia Costa [1] ; da Silva, Thiago Aparecido [1] ; Oliveira, Aline Ferreira [1] ; Freitas, Mateus Silveira [1] ; Hanna, Ebert Seixas [1] ; Almeida, Igor C. [2] ; Gay, Nicholas J. [3] ; Roque-Barreira, Maria Cristina [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol Bioagentes Patogen, Sao Paulo - Brazil
[2] Univ Texas El Paso, Dept Biol Sci, BBRC, El Paso, TX 79968 - USA
[3] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW - England
Total Affiliations: 3
Document type: Journal article
Source: PLoS Neglected Tropical Diseases; v. 8, n. 12 DEC 2014.
Web of Science Citations: 17
Abstract

Background: Paracoccin (PCN) is an N-acetylglucosamine-binding lectin from the human pathogenic fungus Paracoccidioides brasiliensis. Recombinant PCN (rPCN) induces a T helper (Th) 1 immune response when prophylactically administered to BALB/c mice, protecting them against subsequent challenge with P. brasiliensis. In this study, we investigated the therapeutic effect of rPCN in experimental paracoccidioidomycosis (PCM) and the mechanism accounting for its beneficial action. Methodology/Principal Findings: Four distinct regimens of rPCN administration were assayed to identify which was the most protective, relative to vehicle administration. In all rPCN-treated mice, pulmonary granulomas were less numerous and more compact. Moreover, fewer colony-forming units were recovered from the lungs of rPCN-treated mice. Although all therapeutic regimens of rPCN were protective, maximal efficacy was obtained with two subcutaneous injections of 0.5 mg rPCN at 3 and 10 days after infection. The rPCN treatment was also associated with higher pulmonary levels of IL-12, IFN-gamma, TNF-alpha, nitric oxide (NO), and IL-10, without IL-4 augmentation. Encouraged by the pulmonary cytokine profile of treated mice and by the fact that in vitro rPCN-stimulated macrophages released high levels of IL-12, we investigated the interaction of rPCN with Toll-like receptors (TLRs). Using a reporter assay in transfected HEK293T cells, we verified that rPCN activated TLR2 and TLR4. The activation occurred independently of TLR2 heterodimerization with TLR1 or TLR6 and did not require the presence of the CD14 or CD36 co-receptors. The interaction between rPCN and TLR2 depended on carbohydrate recognition because it was affected by mutation of the receptor's N-glycosylation sites. The fourth TLR2 N-glycan was especially critical for the rPCN-TLR2 interaction. Conclusions/Significance: Based on our results, we propose that PCN acts as a TLR agonist. PCN binds to N-glycans on TLRs, triggers regulated Th1 immunity, and exerts a therapeutic effect against P. brasiliensis infection. (AU)

FAPESP's process: 12/20809-6 - Characterization of Toxoplasma gondii microneme proteins interaction with TLR2 glycans
Grantee:Flávia Costa Mendonça Natividade
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/14161-6 - Immunomodulation by Paracoccin: mechanisms of protection conferred against experimental paracoccidioidomycosis
Grantee:Mateus Silveira Freitas
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/09611-0 - Effect of lectin ArtinM on murine CD4+ T and CD8+ T cells
Grantee:Thiago Aparecido da Silva
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 10/01112-9 - Evaluation of paracoccin interaction with cell receptors of innate immunity, in order to determine its role in the acute phase of paracoccidioidomycosis
Grantee:Ana Claudia Paiva Alegre
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/05359-0 - Distribution of paracoccin on the surface of Paracoccidioides brasiliensis and interaction with innate immunity cell receptors: role in the acute phase of paracoccidioidomycosis
Grantee:Aline Ferreira de Oliveira Pereira
Support type: Scholarships in Brazil - Post-Doctorate