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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Melanin Photosensitization and the Effect of Visible Light on Epithelial Cells

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Author(s):
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Chiarelli-Neto, Orlando [1] ; Ferreira, Alan Silva [1] ; Martins, Waleska Kerllen [1] ; Pavani, Christiane [1] ; Severino, Divinomar [1] ; Faiao-Flores, Fernanda [2] ; Maria-Engler, Silvya Stuchi [2] ; Aliprandini, Eduardo [3] ; Martinez, Glaucia R. [3] ; Di Mascio, Paolo [1] ; Medeiros, Marisa H. G. [1] ; Baptista, Mauricio S. [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Dept Quim, Inst Quim, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Analises Clin, BR-09500900 Sao Paulo - Brazil
[3] Univ Fed Parana, Setor Ciencias Biol, Dept Bioquim & Biol Mol, BR-80060000 Curitiba, Parana - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PLoS One; v. 9, n. 11 NOV 18 2014.
Web of Science Citations: 44
Abstract

Protecting human skin from sun exposure is a complex issue that involves unclear aspects of the interaction between light and tissue. A persistent misconception is that visible light is safe for the skin, although several lines of evidence suggest otherwise. Here, we show that visible light can damage melanocytes through melanin photosensitization and singlet oxygen (O-1(2)) generation, thus decreasing cell viability, increasing membrane permeability, and causing both DNA photo-oxidation and necro-apoptotic cell death. UVA (355 nm) and visible (532 nm) light photosensitize O-1(2) with similar yields, and pheomelanin is more efficient than eumelanin at generating O-1(2) and resisting photobleaching. Although melanin can protect against the cellular damage induced by UVB, exposure to visible light leads to pre-mutagenic DNA lesions (i.e., Fpg- and Endo III-sensitive modifications); these DNA lesions may be mutagenic and may cause photoaging, as well as other health problems, such as skin cancer. (AU)

FAPESP's process: 10/08796-0 - Photo-irradiation and cellular damage: correlation between the amount of reactive species and the response of epithelial cells
Grantee:Orlando Chiarelli Neto
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/07937-8 - Redoxome - Redox Processes in Biomedicine
Grantee:Ohara Augusto
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 12/50680-5 - Photosensitization in life sciences
Grantee:Mauricio da Silva Baptista
Support type: Research Projects - Thematic Grants