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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Monoclonal antibodies to heat shock protein 60 induce a protective immune response against experimental Paracoccidioides lutzii

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Author(s):
Thomaz, Luciana [1] ; Nosanchuk, Joshua D. [2, 3] ; Rossi, Diego C. P. [1] ; Travassos, Luiz R. [4] ; Taborda, Carlos P. [5]
Total Authors: 5
Affiliation:
[1] Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
[2] Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10467 - USA
[3] Albert Einstein Coll Med, Dept Med, Bronx, NY 10467 - USA
[4] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil
[5] Univ Sao Paulo, Lab Med Mycol, IMTSP LIM53, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Microbes and Infection; v. 16, n. 9, p. 788-795, SEP 2014.
Web of Science Citations: 6
Abstract

Paracoccidioidomycosis (PCM) is an endemic mycosis in Latin America. PCM is primarily caused by Paracoccidioides brasiliensis and less frequently by the recently described, closely related species Paracoccidioides lutzii. Current treatment requires protracted administration of systemic antibiotics and relapses may frequently occur despite months of initial therapy. Hence, there is a need for innovative approaches to treatment. In the present study we analyzed the impact of two monoclonal antibodies (mAbs) generated against Heat Shock 60 (Hsp60) from Histoplasma capsulatum on the interactions of P. lutzii with macrophages and on the experimental P. lutzii infection. We demonstrated that the Hsp60-binding mAbs labeled P. lutzii yeast cells and enhanced their phagocytosis by macrophage cells. Treatment of mice with the mAbs to Hsp60 before infection reduced the pulmonary fungal burden as compared to mice treated with irrelevant mAb. Hence, mAbs raised to H. capsulatum Hsp60 are protective against P. lutzii, including mAb 7B6 which was non-protective against H. capsulatum, suggesting differences in their capacity to bind to these fungi and to be recognized by macrophages. These findings indicate that mAbs raised to one dimorphic fungus may be therapeutic against additional dimorphic fungi, but also suggests that biological differences in diseases may influence whether a mAb is beneficial or harmful. (C) 2014 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. (AU)

FAPESP's process: 11/17267-4 - Evaluation of a vaccine based on peptide-P10 and the therapeutic effect when administrate in combination with antifungal drugs in the fibrose experimental murine model induzed with Paracoccodioides brasiliensis conidia
Grantee:Carlos Pelleschi Taborda
Support Opportunities: Regular Research Grants
FAPESP's process: 13/18655-3 - Paracoccidioides lutzii: development of a therapeutic vaccine from specific antigens for use in vaccine combined with the P10 peptide P. brasiliensis
Grantee:Carlos Pelleschi Taborda
Support Opportunities: Regular Research Grants