Poly(L-lactic acid) membranes: Absence of genotoxic hazard and potential for drug delivery

Author(s)

Uzun, Nelson ^[1] ; Martins, Thomas Duzzi ^[2] ; Teixeira, Gabriella Machado ^[2] ; Cunha, Nayanne Larissa ^[2] ; Oliveira, Rogerio Belle ^[1] ; Nassar, Eduardo Jose ^[2] ; dos Santos, Raquel Alves ^[2]

Affiliation

^[1] Pontificia Univ Catolica Rio Grande do Sul, Dept Surg, Porto Alegre, RS - Brazil

^[2] Univ Franca, Lab Genet & Mol Biol, BR-14404600 Franca, SP - Brazil

• Document type: Journal article
• Source: Toxicology Letters; v. 232, n. 2, p. 513-518, JAN 22 2015.
• Web of Science Citations: 12

Abstract

The use of poly(L-lactic acid) (PLA) has been considered an important alternative for medical devices once this polyester presents biomechanical, optical and biodegradable properties. Moreover, the use of PLA results in less inflammatory reactions and more recently it has been proposed its application in drug delivery systems. Genotoxicological evaluations are considered part of the battery assays in toxicological analysis. Considering the wide applications of PLA, the present work evaluated the potential cytotoxic and genotoxic effects of PLA in CHO-K1 cells, as well as its physicochemical properties. No cytotoxic effects of PLA were detected by colorimetric tetrazolium assay (XTT) analysis, and the clonogenic survival assay showed that PLA did not disrupt the replicative cell homeostasis, neither exhibited genotoxic effects as evidenced by comet and micronucleus assays. Thermogravimetric properties of PLA were not altered after contact with cells and this film exhibited ability in absorb and release Europium(III) complex. All these data suggest genotoxicological safety of PLA for further applications in drug delivery systems. (c) 2014 Elsevier Ireland Ltd. All rights reserved. (AU)

• FAPESP's process: 12/01465-4 - In vitro assessment of the cytotoxic and genotoxic effects of absorbable poly-L-lactic acid-derived polymer employed for fabrication of surgery and bucomaxilofacial Traumatology materials
• Grantee: Thomás Duzi Martins
• Support type: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 11/17935-7 - Assessment of the intracellular pathways modulated by rosmarinic acid and (-)-hinokinin in response to genotoxic stress
• Grantee: Raquel Alves dos Santos
• Support type: Regular Research Grants
• FAPESP's process: 11/21574-0 - Assessment of modulatory effects of rosmarinic acid and (-)- hinokinin on cellular proliferation, death and DNA repair in response to genotoxic stress on neoplastic breast cell lines
• Grantee: Gabriella Machado Teixeira
• Support type: Scholarships in Brazil - Scientific Initiation