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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Solid lipid nanoparticles as nucleic acid delivery system: Properties and molecular mechanisms

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Author(s):
De Jesus, Marcelo B. [1, 2] ; Zuhorn, Inge S. [2]
Total Authors: 2
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Inst Biol, Campinas, SP - Brazil
[2] Univ Groningen, Univ Med Ctr Groningen, Dept Cell Biol, NL-9713 AV Groningen - Netherlands
Total Affiliations: 2
Document type: Review article
Source: JOURNAL OF CONTROLLED RELEASE; v. 201, p. 1-13, MAR 10 2015.
Web of Science Citations: 45
Abstract

Solid lipid nanoparticles (SLNs) have been proposed in the 1990s as appropriate drug delivery systems, and ever since they have been applied in a wide variety of cosmetic and pharmaceutical applications. In addition, SLNs are considered suitable alternatives as carriers in gene delivery. Although important advances have been made in this particular field, fundamental knowledge of the underlying mechanisms of SLN-mediated gene delivery is conspicuously lacking, an imperative requirement in efforts aimed at further improving their efficiency. Here, we address recent advances in the use of SLNs as platform for delivery of nucleic acids as therapeutic agents. In addition, we will discuss available technology for conveniently producing SLNs. In particular, we will focus on underlying molecular mechanisms by which SLNs and nucleic acids assemble into complexes and how the nucleic acid cargo may be released intracellularly. In discussing underlying mechanisms, we will, when appropriate, refer to analogous studies carried outwith systems based on cationic lipids and polymers, that have proven useful in the assessment of structure-function relationships. Finally, we will give suggestions for improving SLN-based gene delivery systems, by pointing to alternative methods for SLNplex assembly, focusing on the realization of a sustained nucleic acid release. (C) 2015 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 12/01038-9 - Lipid nanoparticles as a Co-Delivery system for genes and drugs: development, cellular processing and biological activity in cancer cells
Grantee:Marcelo Bispo de Jesus
Support Opportunities: Scholarships in Brazil - Post-Doctoral