Immunomodulatory action of Copaifera spp oleoresins on cytokine production by human monocytes

Author(s):Show less -

Santiago, Karina Basso ^[1] ; Conti, Bruno Jose ^[1] ; Murbach Teles Andrade, Bruna Fernanda ^[1] ; Mangabeira da Silva, Jonas Joaquim ^[2] ; Ghislain Rogez, Herve Louis ^[3] ; Crevelin, Eduardo Jose ^[4] ; Beraldo de Moraes, Luiz Alberto ^[4] ; Veneziani, Rodrigo ^[5] ; Ambrosio, Sergio Ricardo ^[5] ; Bastos, Jairo Kenupp ^[2] ; Sforcin, Jose Mauricio ^[1]

Affiliation

^[1] UNESP, Dept Microbiol & Immunol, Biosci Inst, BR-18618970 Botucatu, SP - Brazil

^[2] Univ Sao Paulo, Dept Pharmaceut Sci, Sch Pharmaceut Sci, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Fed Univ Para, Sch Food Engeneering, Inst Technol, BR-66095780 Belem, Para - Brazil

^[4] Univ Sao Paulo, Dept Chem, Sch Phylosophy Sci & Languages, BR-14040901 Ribeirao Preto, SP - Brazil

^[5] Univ Franca, BR-14404600 Franca, SP - Brazil

• Document type: Journal article
• Source: BIOMEDICINE & PHARMACOTHERAPY; v. 70, p. 12-18, MAR 2015.
• Web of Science Citations: 17

Abstract

Copaifera spp oleoresins have been used in folk medicine for centuries; nevertheless, its immunomodulatory action has not been investigated. Thus, the goal of this study was to characterize different oleoresins and to verify their action on human monocytes regarding pro-and anti-inflammatory cytokine production (TNF-alpha and IL-10, respectively). The chemical composition of Brazilian Copaifera reticulata, Copaifera duckey and Copaifera multijuga oleoresins was analyzed by HPLC-MS. Cell viability was assessed by MTT method after incubation of cells with Copaifera spp. Noncytotoxic concentrations of oleoresins were incubated with human monocytes from healthy donors, and cytokine production was determined by ELISA. HPLC-MS analysis for terpenes allowed the identification of six diterpene acids and one sesquiterpene acid. Oleoresins exerted no cytotoxic effects on human monocytes. All oleoresins had a similar profile: LPS-induced TNF-alpha production was maintained by oleoresins, while a significant inhibitory action on IL-10 production was seen. Copaifera oleoresins seemed to exert an activator profile on human monocytes without affecting cell viability. Such effect may be due to the presence of either diterpene or sesquiterpene acids; however, further studies are necessary to determine the involvement of such compounds in Copaifera immunomodulatory effects. (C) 2015 Elsevier Masson SAS. All rights reserved. (AU)

• FAPESP's process: 11/13630-7 - Chemical and pharmacological validation of extracts and active compounds of Copaifera species
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants