Association of mesenchymal stem cells and osteoblasts for bone repair

Author(s)

Santos, Thiago de Santana ^[1] ; Flores Abuna, Rodrigo Paolo ^[1] ; Lopes, Helena Bacha ^[1] ; Goncalves de Almeida, Adriana Luisa ^[1] ; Beloti, Marcio Mateus ^[1] ; Rosa, Adalberto Luiz ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Cell Culture Lab, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Dept Oral & Maxillofacial Surg & Periodontol, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: REGENERATIVE MEDICINE; v. 10, n. 2, p. 127-133, 2015.
• Web of Science Citations: 6

Abstract

Aim: We tested the hypothesis that the association of bone marrow mesenchymal stem cells (MSCs) and osteoblasts (OBs) optimize bone repair. Materials \& Methods: MSCs were cultured in growth or osteogenic medium and seeded into gelatin sponge prior to implantation. Defects were created into rat calvariae and implanted with gelatin sponge without cells, with MSCs, with OBs and with association of MSCs and OBs. Histological analysis and micro-CT-based histomorphometry were carried out after 4 weeks. Results: Increased bone formation was observed in defects treated with cells and bone volume was greater in defects treated with either OBs or MSCs/OBs. Conclusion: Association of MSCs and OBs did not increase the process of bone repair compared with cell-based therapy using either MSCs or OBs alone. (AU)

• FAPESP's process: 11/00919-9 - Effect of cell therapy associating mesenchymal stem cells and osteoblasto on bone repair
• Grantee: Thiago de Santana Santos
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/00617-2 - Effect of cell therapy associating mesenchymal stem cells and osteoblasts on bone repair
• Grantee: Adalberto Luiz Rosa
• Support Opportunities: Regular Research Grants