| Full text | |
| Author(s): |
Rampazo, Eline V.
[1]
;
Amorim, Kelly N. S.
[1]
;
Yamamoto, Marcio M.
[1]
;
Panatieri, Raquel Hoffmann
[1]
;
Rodrigues, Mauricio M.
[2, 3]
;
Boscardin, Silvia B.
[2, 1]
Total Authors: 6
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Parasitol, Lab Antigen Targeting Dendrit Cells, Sao Paulo - Brazil
[2] Natl Inst Sci & Technol Vaccines, Belo Horizonte, MG - Brazil
[3] Univ Fed Sao Paulo, CTCMol, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | PLoS One; v. 10, n. 2 FEB 13 2015. |
| Web of Science Citations: | 11 |
| Abstract | |
Targeting antigens to dendritic cells (DCs) by using hybrid monoclonal antibodies (mAbs) directed against DC receptors is known to improve activation and support long-lasting T cell responses. In the present work, we used the mAb alpha DEC205 fused to the Trypanosoma cruzi amastigote surface protein 2 (ASP-2) to identify a region of this protein recognized by specific T cells. The hybrid alpha DEC-ASP2 mAb was successfully generated and preserved its ability to bind the DEC205 receptor. Immunization of BALB/c mice with the recombinant mAb in the presence of polyriboinosinic: polyribocytidylic acid (poly (I:C)) specifically enhanced the number of IFN-gamma producing cells and CD4+ T cell proliferation when compared to mice immunized with a mAb without receptor affinity or with the non-targeted ASP-2 protein. The strong immune response induced in mice immunized with the hybrid alpha DEC-ASP2 mAb allowed us to identify an ASP-2-specific CD4+ T cell epitope recognized by the BALB/c MHCII haplotype. We conclude that targeting parasite antigens to DCs is a useful strategy to enhance T cell mediated immune responses facilitating the identification of new T-cell epitopes. (AU) | |
| FAPESP's process: | 07/08648-9 - Recombinant antigen targeting to dendritic cells in vivo: a new strategy for the development of vaccines |
| Grantee: | Silvia Beatriz Boscardin |
| Support Opportunities: | Research Grants - Young Investigators Grants |