Development and characterization of microsatellite markers for Vriesea simplex (Bromeliaceae) and cross-amplification in other species of Bromeliaceae

Author(s)

Neri, Jordana ^[1] ; Nazareno, Alison Goncalves ^[2] ; Wendt, Tania ^[3] ; Palma-Silva, Clarisse ^[4]

Affiliation

^[1] Univ Fed Rio de Janeiro, Museu Nacl, Programa Posgrad Bot, BR-20940040 Rio De Janeiro, RJ - Brazil

^[2] Univ Sao Paulo, Inst Biociencias, BR-05508090 Sao Paulo, SP - Brazil

^[3] Univ Fed Rio de Janeiro, Inst Biol, Dept Bot, BR-21941590 Rio De Janeiro, RJ - Brazil

^[4] Univ Estadual Paulista, Programa Posgrad Ecol & Biodiversidade, BR-13506900 Rio Claro, SP - Brazil

• Document type: Journal article
• Source: Biochemical Systematics and Ecology; v. 58, p. 34-37, FEB 2015.
• Web of Science Citations: 9

Abstract

Ten polymorphic microsatellite loci were isolated and characterized for Vriesea simplex epiphytic and endemic species from the Brazilian Atlantic Rainforest. The number of alleles per locus ranged from 2 to 16. The observed and expected heterozygosity ranged from 0.000 to 0.414 and 0.068 to 0.795, respectively. All loci departed significantly from Hardy-Weinberg equilibrium. Linkage disequilibrium was not detected in any pair of loci. Transferability of 10 loci was positive across ten other Bromeliaceae species. The loci will be used for studying population genetic structure, mating system variation, and the origin and maintenance of reproductive barriers to gene exchange among sympatric Vriesea species. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 09/52725-3 - Speciation, reproductive isolation, and population genetics in species of Bromeliaceae family: implications for taxonomy, evolution and conservation
• Grantee: Clarisse Palma da Silva
• Support Opportunities: BIOTA-FAPESP Program - Young Investigators Grants