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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular diagnosis of cryptococcal meningitis in cerebrospinal fluid: comparison of primer sets for Cryptococcus neoformans and Cryptococcus gattii species complex

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Martins, Marilena dos Anjos [1, 2] ; Santos Brighente, Kate Bastos [1] ; de Matos, Terezinha Aparecida [3] ; Vidal, Jose Ernesto [4] ; Carnietto de Hipolito, Daise Damaris [2] ; Pereira-Chioccola, Vera Lucia [1]
Total Authors: 6
[1] Adolfo Lutz Inst, Lab Biol Mol Parasitas & Fungos, BR-01246902 Sao Paulo - Brazil
[2] Adolfo Lutz Inst, Nucleo Micol, BR-01246902 Sao Paulo - Brazil
[3] Inst Infectol Emilio Ribas, Lab Liquido Cefalorraquiano, Sao Paulo - Brazil
[4] Inst Infectol Emilio Ribas, Dept Neurol, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Brazilian Journal of Infectious Diseases; v. 19, n. 1, p. 62-67, JAN-FEB 2015.
Web of Science Citations: 4

Aim: This study evaluated the use of polymerase chain reaction for cryptococcal meningitis diagnosis in clinical samples. Materials and methods: The sensitivity and specificity of the methodology were evaluated using eight Cryptococcus neoformans/C. gattii species complex reference strains and 165 cerebrospinal fluid samples from patients with neurological diseases divided into two groups: 96 patients with cryptococcal meningitis and AIDS; and 69 patients with other neurological opportunistic diseases (CRL/AIDS). Two primer sets were tested (CN4-CN5 and the multiplex CNa70S-CNa70A/CNb49S-CNb-49A that amplify a specific product for C. neoformans and another for C. gattii). Results: CN4-CN5 primer set was positive in all Cryptococcus standard strains and in 94.8% in DNA samples from cryptococcal meningitis and AIDS group. With the multiplex, no 448-bp product of C. gattii was observed in the clinical samples of either group. The 695 bp products of C. neoformans were observed only in 64.6% of the czyptococcal meningitis and AIDS group. This primer set was negative for two standard strains. The specificity based on the negative samples from the CTL/AIDS group was 98.5% in both primer sets. Conclusions: These data suggest that the CN4/CN5 primer set was highly sensitive for the identification of C. neoformans/C. gotta species complex in cerebrospinal fluid samples from patients with clinical suspicion of cryptococcal meningitis. (C) 2014 Elsevier Editora Ltda. Este e um artigo Open Access sob a licenca de CC BY-NC-ND (AU)

FAPESP's process: 11/13939-8 - Study of a group of fungal and parasitic infections aiming the improvement of techniques of characterization and immune-molecular diagnosis
Grantee:Vera Lúcia Pereira Chioccola
Support type: Regular Research Grants