Enriched inorganic compounds in diesel exhaust particles induce mitogen-activated protein kinase activation, cytoskeleton instability, and cytotoxicity in human bronchial epithelial cells

Author(s):Show less -

Seriani, Robson ^[1] ; Junqueira, Mara S. ^[2] ; Carvalho-Sousa, Claudia E. ^[3] ; Arruda, Alessandra Ct. ^[4] ; Martinez, Diana ^[5] ; Alencar, Adriano M. ^[5] ; Garippo, Ana L. ^[6] ; Brito, Jose Mara ^[1] ; Martins, Milton A. ^[4] ; Saldiva, Paulo H. N. ^[1] ; Negri, Elnara M. ^[1] ; Mauad, Thais ^[1] ; Macchione, Mariangela ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Med, Dept Pathol, Lab Expt Air Pollut LIM05, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Cent Biotety Lab, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Physiol, Inst Biosci, Lab Chronopharmacol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Med, Dept Med, Expt Therapeut Lab, BR-05508 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Inst Phys, Lab Microrheol & Mol Physiol, Sao Paulo - Brazil

^[6] Univ Sao Paulo, PREMIUM Confocal Microscopy Core Facil, BR-05508 Sao Paulo - Brazil

• Document type: Journal article
• Source: EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY; v. 67, n. 4, p. 323-329, APR 2015.
• Web of Science Citations: 4

Abstract

This study assessed the effects of the diesel exhaust particles on ERR and JNK MAPKs activation, cell rheology (viscoelasticity), and cytotoxicity in bronchial epithelial airway cells (BEAS-2B). Crude DEP and DEP after extraction with hexane (DEP/HEX) were utilized. The partial reduction of some DEP/HEX organics increased the biodisponibility of many metallic elements. JNK and ERR were activated simultaneously by crude DEP with no alterations in viscoelasticity of the cells. Mitochondrial activity, however, revealed a decrease through the MIT assay. DEP/HEX treatment increased viscoelasticity and cytotoxicity (membrane damage), and also activated JNK. Our data suggest that the greater bioavailability of metals could be involved in JNK activation and, consequently, in the reduction of fiber coherence and increase in the viscoelasticity and cytotoxicity of BEAS cells. The adverse findings detected after exposure to crude DEP and to DEP/HEX reflect the toxic potential of diesel compounds. Considering the fact that the cells of the respiratory epithelium are the first line of defense between the body and the environment, our data contribute to a better understanding of the pathways leading to respiratory cell injury and provide evidence for the onset of or worsening of respiratory diseases caused by inorganic compounds present in DEP. (C) 2015 Elsevier GmbH. All rights reserved. (AU)