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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

L-glutamine Supplementations Enhance Liver Glutamine-Glutathione Axis and Heat Shock Factor-1 Expression in Endurance-Exercise Trained Rats

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Author(s):
Petry, Eder Ricardo [1] ; Cruzat, Vinicius Fernandes [1, 2] ; Heck, Thiago Gomes [3] ; Homem de Bittencourt, Jr., Paulo Ivo [4] ; Tirapegui, Julio [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Food Sci & Expt Nutr, Sao Paulo - Brazil
[2] Curtin Univ, Biosci Res Precinct, CHIRI, Sch Biomed Sci, Perth, WA 6845 - Australia
[3] Univ Northwest Rio Grande Sul State, Ijui, RS - Brazil
[4] Univ Fed Rio Grande do Sul, Inst Basic Hlth Sci, Dept Physiol, Porto Alegre, RS - Brazil
Total Affiliations: 4
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM; v. 25, n. 2, p. 188-197, APR 2015.
Web of Science Citations: 12
Abstract

Liver L-glutamine is an important vehicle for the transport of ammonia and intermediary metabolism of amino acids between tissues, particularly under catabolic situations, such as high-intensity exercise. Hence, the aim of this study was to investigate the effects of oral supplementations with L-glutamine in its free or dipeptide forms (with L-alanine) on liver glutamine-glutathione (GSH) axis, and 70 kDa heat shock proteins (HSP70)/heat shock transcription factor 1 (HSF1) expressions. Adult male Wistar rats were 8-week trained (60 min/day, 5 days/week) on a treadmill. During the last 21 days, the animals were daily supplemented with 1 g of L-glutamine/kg body weight per day in either 1-alanyl-L-glutamine dipeptide (DIP) form or a solution containing L-glutamine and 1-alanine in their free forms (GLN+ALA) or water (controls). Exercise training increased cytosolic and nuclear HSF1 and HSP70 expression, as compared with sedentary animals. However, both DIP and GLN+ALA supplements enhanced HSF1 expression (in both cytosolic and nuclear fractions) in relation to exercised controls. Interestingly, HSF1 rises were not followed by enhanced HSP70 expression. DIP and GLN+ALA supplements increased plasma glutamine concentrations (by 62% and 59%, respectively) and glutamine to glutamate plasma ratio in relation to trained controls. This was in parallel with a decrease in plasma ammonium levels. Supplementations increased liver GSH (by 90%), attenuating the glutathione disulfide (GSSG) to GSH ratio, suggesting a redox state protection. In conclusion, oral administration with DIP and GLN+ALA supplements in endurance-trained rats improve liver glutamine-GSH axis and modulate HSF1 pathway. (AU)