Chromobacterium amazonense sp nov isolated from water samples from the Rio Negro, Amazon, Brazil

Author(s)

Afonso Menezes, Claudia Beatriz ^{[1, 2]} ; Tonin, Mariana Ferreira ^[3] ; Alves Correa, Daniele Bussioli ^[3] ; Parma, Marcia ^[4] ; de Melo, Itamar Soares ^[4] ; Zucchi, Tiago Domingues ^[4] ; Lanza Destefano, Suzete Aparecida ^[3] ; Fantinatti-Garboggini, Fabiana ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Programa Posgrad Interunidades Biotecnol, BR-05508900 Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr, BR-13083218 Campinas, SP - Brazil

^[3] Secretaria Agr & Abastecimento, Ctr Expt, Inst Biol, Lab Bacteriol Vegetal, BR-13092543 Campinas, SP - Brazil

^[4] Embrapa Meio Ambiente, BR-13820000 Jaguariuna, SP - Brazil

• Document type: Journal article
• Source: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY; v. 107, n. 4, p. 1057-1063, APR 2015.
• Web of Science Citations: 8

Abstract

The taxonomic position of a bacterium isolated from water samples from the Rio Negro, in Amazon, Brazil, was determined by using a polyphasic approach. The organism formed a distinct phyletic line in the Chromobacterium 16S rRNA gene tree and had chemotaxonomic and morphological properties consistent with its classification in this genus. It was found to be closely related to Chromobacterium vaccinii DSM 25150(T) (98.6 % 16S rRNA gene similarity) and shared 98.5 % 16S rRNA gene similarity with Chromobacterium piscinae LGM 3947(T). DNA-DNA relatedness studies showed that isolate CBMAI 310(T) belongs to distinct genomic species. The isolate was readily distinguished from the type strain of these species using a combination of phenotypic and chemotaxonomic properties. Thus, based on genotypic and phenotypic data, it is proposed that isolate CBMAI 310(T) (=DSM 26508(T)) be classified in the genus Chromobacterium as the type strain of a novel species, namely, Chromobacterium amazonense sp. nov. (AU)