| Full text | |
| Author(s): |
Fernandez-Nunez, Eutimio Gustavo
[1, 2]
;
de Rezende, Alexandre Goncalves
[3]
;
Pradella Puglia, Ana Lia
[3]
;
Leme, Jaci
[4]
;
Boldorini, Vera Lucia Lopes
[3]
;
Caricati, Celso Pereira
[4]
;
Tonso, Aldo
[1]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Lab Celulas Anim, Dept Engn Quim, Escola Politecn, BR-05508900 Sao Paulo, SP - Brazil
[2] Univ Estadual Julio de Mesquita Filho, Dept Ciencias Biol, BR-19806900 Assis, SP - Brazil
[3] Inst Butantan, Lab Imunol Viral, BR-05503900 Sao Paulo, SP - Brazil
[4] Inst Butantan, Lab Especial Pesquisa & Desenvolvimento Imunol Ve, BR-05503900 Sao Paulo, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Biotechnology Letters; v. 37, n. 6, p. 1153-1163, JUN 2015. |
| Web of Science Citations: | 0 |
| Abstract | |
To assess the expression of rabies virus G-glycoprotein (RVGP) expression using Semliki Forest virus as a vector in combination with BHK-21 cells cultured in suspension. A multilevel factorial design was used to quantify effects of temperature (33-37 A degrees C), fresh medium addition after the viral adsorption step (100-200 % with respect to the initial cell suspension volume before infection) and harvest time (8-40 h) on RVGP production. Experimental runs were performed in 24-well cell culture plates at a multiplicity of infection (MOI) of 16. An additional experiment in spinner-flask was performed at MOI of 9, using the optimal conditions determined in cell culture plates. Values for temperature, fresh medium addition and harvest time of 33 A degrees C, 100 % and 16 h, respectively, ensured the optimal RVGP production in culture plates. The volumetric yield (239 ng ml(-1)) in these conditions was higher than that reported previously for adherent cell culture. In spinner-flasks, the volumetric yield was improved (559 ng ml(-1)). These results establish the basis for designing bioprocess to produce RVGP. (AU) | |