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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Maternal Protein Restriction Increases Respiratory and Sympathetic Activities and Sensitizes Peripheral Chemoreflex in Male Rat Offspring

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Author(s):
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de Brito Alves, Jose L. [1] ; Nogueira, Viviane O. [1] ; Cavalcanti Neto, Marinaldo P. [2, 3] ; Leopoldino, Andreia M. [2, 3] ; Curti, Carlos [2, 3] ; Colombari, Debora S. A. [4] ; Colombari, Eduardo [4] ; Wanderley, Almir G. [5] ; Leandro, Carol G. [1] ; Zoccal, Daniel B. [5] ; Costa-Silva, Joao H. [1]
Total Authors: 11
Affiliation:
[1] Univ Fed Pernambuco, Dept Phys Educ & Sport Sci, Vitoria De Santo Antao, PE - Brazil
[2] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Phys & Chem, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Clin Anal Toxicol & Food Sci, Sao Paulo - Brazil
[4] Sao Paulo State Univ, Sch Dent Araraquara, Dept Physiol & Pathol, Sao Paulo - Brazil
[5] Univ Fed Pernambuco, Dept Physiol & Pharmacol, Recife, PE - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of Nutrition; v. 145, n. 5, p. 907-914, MAY 2015.
Web of Science Citations: 17
Abstract

Background: Maternal protein restriction in rats increases the risk of adult offspring arterial hypertension through unknown mechanisms. Objectives: The aims of the study were to evaluate the effects of a low-protein (LP) diet during pregnancy and lactation on baseline sympathetic and respiratory activities and peripheral chemoreflex sensitivity in the rat offspring. Methods: Wistar rat dams were fed a control {[}normal-protein (NP); 17% protein] or an LP (8% protein) diet during pregnancy and lactation, and their male offspring were studied at 30 d of age. Direct measurements of baseline arterial blood pressure (ABP), heart rate (HR), and respiratory frequency (Rf) as well as peripheral chemoreflex activation (potassium cyanide: 0.04%) were recorded in pups while they were awake. In addition, recordings of the phrenic nerve (PN) and thoracic sympathetic nerve (tSN) activities were obtained from the in situ preparations. Hypoxia-inducible factor l alpha (HIF-1 alpha) expression was also evaluated in carotid bifurcation through a Western blotting assay. Results: At 30 d of age, unanesthetized LP rats exhibited enhanced resting Rf (P = 0.001) and similar ABP and HR compared with the NP rats. Despite their similar baseline ABP values, LP rats exhibited augmented low-frequency variability (similar to 91%; P = 0.01). In addition, the unanesthetized LP rats showed enhanced pressor (P = 0.01) and tachypnoeic (P = 0.03) responses to peripheral chemoreflex activation. The LP rats displayed elevated baseline tSN activity (similar to 86%; P= 0.02) and PN burst frequency (45%; P= 0.01) and amplitude (53%; P= 0.001) as well as augmented sympathetic (P= 0.01) and phrenic (P= 0.04) excitatory responses to peripheral chemoreflex activation compared with the NP group. Furthermore, LP rats showed an increase of similar to 100% in HIF-1 alpha protein density in carotid bifurcation compared with NP rats. Conclusion: Sympathetic-respiratory overactivity and amplified peripheral chemoreceptor responses, potentially through HIF-1a dependent mechanisms, precede the onset of hypertension in juvenile rats exposed to protein undernutrition during gestation and lactation. (AU)

FAPESP's process: 09/54888-7 - Neural mechanisms involved on chemoreception
Grantee:Eduardo Colombari
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 11/20040-1 - Cardiorespiratory cholinergic function in the nucleus of the solitary tract
Grantee:Débora Simões de Almeida Colombari
Support Opportunities: Regular Research Grants