Evaluation of the progression and treatment of experimental periodontitis in rats subjected to chemotherapy with 5-fluorouracil

Author(s)

Garcia, Valdir Gouveia ^{[1, 2, 3, 4]} ; Noronha Novaes, Vivian Cristina ^{[1, 2]} ; de Almeida, Juliano Milanezi ^{[1, 2]} ; Longo, Mariellen ^{[1, 2]} ; Ervolino, Edilson ^[5] ; Mogami Bomfim, Suely Regina ^[6] ; Theodoro, Leticia Helena ^{[1, 2]}

Affiliation

^[1] Univ Estadual Paulista UNESP, Dept Surg, Sao Paulo - Brazil

^[2] Univ Estadual Paulista UNESP, Div Periodont, Integrated Clin, Sao Paulo - Brazil

^[3] Univ Ctr Educ Fdn Barretos UNIFEB, Barretos, SP - Brazil

^[4] Univ Estadual Paulista UNESP, Fac Odontol Aracatuba, BR-16015050 Aracatuba, SP - Brazil

^[5] Univ Estadual Paulista UNESP, Dept Basic Sci, Sao Paulo - Brazil

^[6] Univ Estadual Paulista UNESP, Dept Clin Surg & Anim Reprod, Sao Paulo - Brazil

• Document type: Journal article
• Source: SUPPORTIVE CARE IN CANCER; v. 23, n. 7, p. 2007-2017, JUL 2015.
• Web of Science Citations: 10

Abstract

This study aimed to evaluate the progression and treatment of experimental periodontitis (EP) in rats treated with 5-fluorouracil (5-FU). One-hundred fifty rats were divided into groups: C-control, EP-EP induction, EP/scaling and root planing (SRP)-EP induction and SRP, 5-FU-systemic treatment with 5-FU, 5-FU/EP-systemic treatment with 5-FU and EP induction, and 5-FU/EP/SRP-systemic treatment with 5-FU, EP induction and SRP. Systemic treatment consisted of the administration of 80 and 40 mg/kg of 5-FU. The animals were euthanised at 7, 15 and 30 days. Histological and immunohistochemical analyses for the detection of the receptor activator of nuclear factor-kappa B ligand (RANKL), osteoprotegerin (OPG) and tartrate-resistant acid phosphatase (TRAP) were performed at the furcation region. A histometric analysis was performed to evaluate the percentage of bone in the furcation region (PBF). In the 5-FU/EP and 5-FU/EP/SRP groups, there was less PBF, greater breakdown of periodontal tissues and increased immunostaining for RANKL compared with the EP and EP/SRP groups. It can be concluded that 5-FU aggravates PE progression, impairs the host response to SRP mechanical therapy and does not seem to cause changes in the healthy periodontium. (AU)

• FAPESP's process: 11/03021-3 - Influence of 5-fluorouracil in the normal periodontal tissues and the evolution of experimentally induced periodontitis
• Grantee: Juliano Milanezi de Almeida
• Support Opportunities: Regular Research Grants