EFFECTS OF Bifidobacterium animalis subsp. lactis HN019 PROBIOTIC ADMINISTRATION ON LIGATURE INDUCED PERIODONTAL DISEASE IN RATS SUBMITTED TO CHEMOTHERAPY

• Grant number: 17/11541-3
• Support type: Regular Research Grants
• Duration: October 01, 2018 - March 31, 2021
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal researcher: Luciana Prado Maia
• Grantee: Luciana Prado Maia
• Home Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil
• Assoc. researchers: Michel Reis Messora

Abstract

Chemotherapy treatment increases the severity of periodontitis and compromises treatment outcome. Probiotics have emerged as an alternative for the treatment of oral pathologies, modulating the host immune response and modifying the bacterial environment. The aim of the presente study is to evaluate the effects of Bifidobacterium animalis subsp. lactis HN019 probiotic administration on experimental periodontitis (EP) progression in rats submitted to chemotherapy (5-fluorouracil - 5-FU) via morphometric, histomorphometric, microtomographic, immunohistochemical, immunoenzymatic and gene expression assays. 80 male rats will be used, devided into the following groups: control (C); treatment only with 5-FU (5FU) (60 and 40 mg/kg); treatment only with PROB (HN019) (daily for 44 days, beginning 30 before EP induction); treatment with 5-FU and PROB (5FU-HN019); only EP (EP) (ligature on inferior first molars, left for 14 days); EP and treatment with 5-FU (PE-5FU); EP and treatment with PROB (PE-HN019) and EP and treatment with 5-FU and PROB (PE-5FU-HN019). All animals will be euthanized 44 days after the beginning of the experiment and oral biofilm, gingival tissue, hemi-mandibules, intestine and feces will be collected. For normal distribution data, Variance Analysis (ANOVA) followed by the post-hoc test Tukey will be used. For data with non-normal distribution Kruskal-Wallis followed by the post-hoc test of Dunn will be used. It is expected with this study to demonstrate that the use of probiotics could be considered as an important tool in periodontal disease prevention in individuals under chemotherapy. (AU)