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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Profile of skin barrier proteins (filaggrin, claudins 1 and 4) and Th1/Th2/Th17 cytokines in adults with atopic dermatitis

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Author(s):
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Batista, D. I. S. [1] ; Perez, L. [1] ; Orfali, R. L. [1] ; Zaniboni, M. C. [1] ; Samorano, L. P. [1] ; Pereira, N. V. [2] ; Sotto, M. N. [1] ; Ishizaki, A. S. [1] ; Oliveira, L. M. S. [2] ; Sato, M. N. [2] ; Aoki, V. [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Sch Med, Dept Dermatol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Lab Med Investigat Dermatol & Immunodeficiency, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY; v. 29, n. 6, p. 1091-1095, JUN 2015.
Web of Science Citations: 46
Abstract

BackgroundAtopic dermatitis (AD) in adults and profile of skin barrier proteins and inflammatory cytokines. ObjectiveEvaluation of the expression of skin barrier proteins such as filaggrin, claudins 1 and 4 and of circulating inflammatory cytokines (Th1/Th2/Th17) in adults with AD. MethodsThirty-three adult patients with AD diagnosed according to the Hanifin \& Rajkacriteria, and 25 healthy controls were enrolled in the study. AD severity was measured by Eczema Area and Severity Index (EASI). Laboratory assays included immunohistochemistry analysis of skin barrier proteins, such as filaggrin, claudins 1 and 4 and interleukin-17 (IL-17) from skin samples and determination of circulating cytokine levels (IL-2, 4, 5, 6, 10, 17A, TNF and IFN-) by flow cytometry (Cytometric Bead Array). ResultsWe observed a reduced expression of filaggrin and claudin 1 in lesional skin of AD patients, when compared to controls. There was an inverse correlation of filaggrin expression and disease severity. In addition, IL-17 expression was enhanced in AD patients. Similarly, higher levels of inflammatory cytokines (IL-2, 5, 6, 10, 17A and IFN-) were found in AD patients. ConclusionOur data reinforce the role of an altered skin barrier in the pathogenesis of AD. Our results show not only reduced expression of filaggrin and claudin 1 in lesional atopic skin but also inverse correlation of filaggrin expression and disease severity. Moreover, elevation of in situ IL-17 and of circulating interleukin levels in AD emphasize the systemic, inflammatory profile of this defective skin barrier dermatosis. (AU)

FAPESP's process: 11/02453-7 - Atopic dermatitis in adults: changes in skin barrier and Th17 and Th22 cell-mediated immune response
Grantee:Valeria Aoki
Support Opportunities: Regular Research Grants