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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Chronic inhibition of brain phospholipase A(2) in adult rats impairs the survival of newborn mature neurons in the hippocampus

Full text
Author(s):
Schaeffer, Evelin L. [1] ; Gattaz, Wagner F. [1]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, Fac Med, Dept & Inst Psychiat, Lab Neurosci LIM 27, BR-05403010 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF NEURAL TRANSMISSION; v. 122, n. 5, p. 619-628, MAY 2015.
Web of Science Citations: 2
Abstract

Adult neurogenesis occurs in the hippocampal dentate gyrus (DG) and lateral ventricles, and includes cell proliferation and neuronal differentiation, maturation and survival. In vitro studies suggest a role for phospholipase A(2) (PLA(2)) in neuronal differentiation/maturation and survival. This study aimed to investigate the effect of in vivo chronic inhibition of brain PLA(2) in adult rats on the number of newborn mature neurons in the DG. Male Wistar rats were injected with BrdU (cell proliferation marker) and 2 weeks later (beginning of neuronal maturation) sham-operated or infused intracerebroventricularly with either vehicle (DMSO in saline) or PLA(2) inhibitor (MAFP dissolved in the vehicle) for 14 days via osmotic minipump. The animals were euthanised 28 days post-BrdU and their brains immunostained for BrdU and BrdU plus NeuN (mature neuronal marker) for analysis of surviving cells. MAFP reduced the number of BrdU ? cells in the ventral DG (p < 0.05 vs. sham; p < 0.01 vs. DMSO) and the number of BrdU(+)NeuN(+) cells in the ventral (p < 0.01 vs. sham and DMSO) and whole DG (p < 0.02 vs. sham and DMSO). There was no effect of MAFP in the dorsal DG. These findings show that chronic PLA(2) inhibition in adult rat hippocampus decreases the number of newborn mature neurons in the ventral DG (reflecting in the whole DG), perhaps by impairing neuronal maturation and survival, and suggest that PLA(2) inhibition reported in the hippocampus of Alzheimer disease subjects might partly contribute to the neurogenic abnormalities found in the DG in this disease. (AU)

FAPESP's process: 09/52825-8 - Neurobiology of Alzheimer's disease: risk markers, prognosis and therapeutic response
Grantee:Wagner Farid Gattaz
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 09/53008-3 - Effects of chronic phospholipase A2 inhibition on the differentiation of new neurons in brain mature cells of adult rats
Grantee:Evelin Lisete Schaeffer
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 10/05442-3 - Effects of chronic phospholipase A2 inhibition on the differentiation of new neurons into mature cells in the brain of adult rats
Grantee:Evelin Lisete Schaeffer
Support Opportunities: Scholarships in Brazil - Young Researchers