Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immunomodulatory Effects of Dietary -glucan and Vitamin C in Nile Tilapia, Oreochromis niloticus L., Subjected to Cold-induced Stress or Bacterial Challenge

Full text
Author(s):
Barros, Margarida M. [1] ; Falcon, Dario R. [1] ; Orsi, Ricardo O. [1] ; Pezzato, Luiz E. [1] ; Fernandes Junior, Ademir C. [1] ; Fernandes Junior, Ary [1] ; de Carvalho, Pedro L. P. F. [1] ; Padovani, Carlos R. [1] ; Guimaraes, Igo G. [2] ; Sartori, Maria M. P. [3]
Total Authors: 10
Affiliation:
[1] Sao Paulo State Univ, Sch Vet Med & Anim Sci, BR-18618970 Botucatu, SP - Brazil
[2] Univ Fed Goias, Aquaculture Res Lab, BR-75801615 Jatai, Go - Brazil
[3] Sao Paulo State Univ, Sch Agr Sci, BR-18618970 Botucatu, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF THE WORLD AQUACULTURE SOCIETY; v. 46, n. 4, p. 363-380, AUG 2015.
Web of Science Citations: 3
Abstract

To determine the dietary -glucan and vitamin C levels that would enable the fish to cope with different stress, 252 tilapia (16.86 +/- 0.24 g) were distributed in thirty six 250-L aquaria and fed experimental diets for 60 d. Diets were supplemented with -glucan (0.1, 0.2, 0.4, and 0.8%) and vitamin C (400 and 600 mg/kg diet) and distributed in a factorial design that included a control diet (0 -glucan/125 mg vitamin C/kg). After growth performance analyses, a group of 108 fish were subjected to cold-induced stress and another to Aeromonas hydrophila infection. Hematological and immunological parameters were determined before and after the stress. Growth performance was not affected. Red blood cells, hematocrit, mean corpuscular volume, total plasma protein, albumin : globulin ratio and leukocytes, lymphocytes, neutrophils, and monocytes were affected (P < 0.05) by stress and/or diets. The control diet resulted in higher post-infection cortisol (P < 0.05). Liver vitamin C was directly proportional to dietary vitamin C (P < 0.05). The production of hydrogen peroxide and nitric oxide was also affected by diets and/or bacterial challenge (P < 0.05). In total, 0.1-0.2% -glucan and 600 mg/kg vitamin C increased fish resistance to stress and 0.8% -glucan resulted in reduced immune responses regardless of the vitamin C supplementation level. (AU)