Rutin administration attenuates myocardial dysfunction in diabetic rats

Author(s)

Guimaraes, Julliano F. C. ^[1] ; Muzio, Bruno P. ^[1] ; Rosa, Camila M. ^[1] ; Nascimento, Andre F. ^[1] ; Sugizaki, Mario M. ^[1] ; Fernandes, Ana A. H. ^[2] ; Cicogna, Antonio C. ^[1] ; Padovani, Carlos R. ^[1] ; Okoshi, Marina P. ^[1] ; Okoshi, Katashi ^{[1, 3]}

Affiliation

^[1] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Internal Med, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, UNESP, Inst Biosci, Dept Chem & Biochem, Botucatu, SP - Brazil

^[3] UNESP, Fac Med Botucatu, Dept Clin Med, BR-18618970 Botucatu, SP - Brazil

• Document type: Journal article
• Source: CARDIOVASCULAR DIABETOLOGY; v. 14, JUL 17 2015.
• Web of Science Citations: 17

Abstract

Background: Oxidative stress plays a major role in diabetic cardiomyopathy pathogenesis. Anti-oxidant therapy has been investigated in preventing or treating several diabetic complications. However, anti-oxidant action on diabetic-induced cardiac remodeling is not completely clear. This study evaluated the effects of rutin, a flavonoid, on cardiac and myocardial function in diabetic rats. Methods: Wistar rats were assigned into control (C, n = 14); control-rutin (C-R, n = 14); diabetes mellitus (DM, n = 16); and DM-rutin (DM-R, n = 16) groups. Seven days after inducing diabetes (streptozotocin, 60 mg/kg, i.p.), rutin was injected intraperitoneally once a week (50 mg/kg) for 7 weeks. Echocardiogram was performed and myocardial function assessed in left ventricular (LV) papillary muscles. Serum insulin concentration was measured by ELISA. Statistics: One-way ANOVA and Tukey's post hoc test. Results: Glycemia was higher in DM than DM-R and C and in DM-R than C-R. Insulin concentration was lower in diabetic groups than controls (C 2.45 +/- 0.67; C-R 2.09 +/- 0.52; DM 0.59 +/- 0.18; DM-R 0.82 +/- 0.21 ng/mL). Echocardiogram showed no differences between C-R and C. DM had increased LV systolic diameter compared to C, and increased left atrium diameter/body weight (BW) ratio and LV mass/BW ratio compared to C and DM-R. Septal wall thickness, LV diastolic diameter/BW ratio, and relative wall thickness were lower in DM-R than DM. Fractional shortening and posterior wall shortening velocity were lower in DM than C and DM-R. In papillary muscle preparation, DM and DM-R presented higher time to peak tension and time from peak tension to 50% relaxation than controls; time to peak tension was lower in DM-R than DM. Under 0.625 and 1.25 mM extracellular calcium concentrations, DM had higher developed tension than C. Conclusion: Rutin attenuates cardiac remodeling and left ventricular and myocardial dysfunction caused by streptozotocin-induced diabetes mellitus. (AU)