Influence of empaglifozin on the heart of rats with myocardial infarction-induced ...
| Grant number: | 14/18685-2 |
| Support type: | Regular Research Grants - Publications - Scientific article |
| Duration: | November 01, 2014 - April 30, 2015 |
| Field of knowledge: | Biological Sciences - Physiology |
| Principal Investigator: | Christiane Malfitano |
| Grantee: | Christiane Malfitano |
| Home Institution: | Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil |
Abstract
Background: Previous research has demonstrated that hyperglycemia may protect the heart against ischemic injury. The aim of the present study was to investigate the association between hyperglycemia and myocardial infarction on cardiovascular autonomic modulation and cardiac oxidative stress profile in rats. Male Wistar rats were divided into: control (C), diabetic (D), myocardial infarcted (MI) and diabetic infarcted rats (DMI). Methods: Diabetes was induced by streptozotocin (STZ, 50mg/Kg) at the beginning of the protocol and MI was induced by left coronary occlusion 15 days after STZ. Thirty days after streptozocin-induced diabetes, cardiovascular autonomic modulation was evaluated by spectral analysis, and oxidative stress profile was determined by antioxidant enzyme activities and superoxide anion, together with protein carbonylation and redox balance of glutathione (GSH/GSSG). Results: The diabetic and infarcted groups showed decreased heart rate variability and vagal modulation (p<0.05); however, sympathetic modulation decreased only in diabetic groups (p<0.05). Sympatho/vagal balance and vascular sympathetic modulation were increased only in the MI group (p<0.05). Diabetes promoted an increase in catalase concentration (p<0.05). Glutathione peroxidase activity was increased only in DMI when compared to the other groups (p<0.05). Superoxide anion and protein carbonylation were increased only in MI group (p<0.05). Cardiac redox balance, as evaluated by GSH/GSSG, was lower in the MI group (p<0.05). Conclusions: These data suggest that hyperglycemia promotes compensatory mechanisms that may offer protection against ischemia, as demonstrated by increased antioxidants, decreased pro-oxidants and protein damage, possibly related to the improvements in both redox balance and sympathetic modulation to the heart. (AU)