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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Direct Protocol for Ambient Mass Spectrometry Imaging on Agar Culture

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Author(s):
Angolini, Celio Fernando F. [1] ; Vendramini, Pedro Henrique [1] ; Araujo, Francisca D. S. [1] ; Araujo, Welington L. [2] ; Augusti, Rodinei [3] ; Eberlin, Marcos N. [1] ; de Oliveira, Luciana Gonzaga [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Inst Chem, Dept Organ Chem, BR-13083970 Campinas, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo, SP - Brazil
[3] Univ Fed Minas Gerais, Dept Chem, BR-31270901 Belo Horizonte, MG - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Analytical Chemistry; v. 87, n. 13, p. 6925-6930, JUL 7 2015.
Web of Science Citations: 20
Abstract

Herein we describe a new protocol that allows direct mass spectrometry imaging (IMS) of agar cultures. A simple sample dehydration leads to a thin solid agar, which enables the direct use of spray-based ambient mass spectrometry techniques. To demonstrate its applicability, metal scavengers siderophores were imaged directly from agar culture of S. wadayamensis, and well resolved and intense images were obtained using both desorption electrospray ionization (DESI) and easy ambient sonic-spray ionization (EAST) with well-defined selective spatial distributions for the free and the metal-bound molecules, providing clues for their roles in cellular metabolism. (AU)

FAPESP's process: 10/51677-2 - Development of new mass spectrometry techniques and their general applications in science: chemistry, biochemistry, material sciences, forensics, medicine, food science, pharmaceutical and veterinary medicine
Grantee:Marcos Nogueira Eberlin
Support Opportunities: Research Projects - Thematic Grants