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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Polymorphisms of the TLR2 and TLR4 genes are associated with risk of gastric cancer in a Brazilian population

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Author(s):
de Oliveira, Juliana Garcia [1] ; Silva, Ana Elizabete [1]
Total Authors: 2
Affiliation:
[1] UNESP Sao Paulo State Univ, Dept Biol, BR-15054000 Sao Paulo, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: WORLD JOURNAL OF GASTROENTEROLOGY; v. 18, n. 11, p. 1235-1242, MAR 21 2012.
Web of Science Citations: 64
Abstract

AIM: To investigate toll-like receptor 2 (TLR2) -196 to -174 del, and TLR4 (+896A/G rs4986790 and +1196C/T rs4986791) polymorphisms at risk of chronic gastritis and gastric cancer in a Brazilian population and association of gastric lesions with risk factors such as smoking, alcohol intake and Helicobacter pylori infection. METHODS: In this case-control study, polymorphism at TLR2 496 to -174 del was investigated by using the polymerase chain reaction (PCR) method, while the PCR-restriction fragment length polymorphism technique was carried out to identify the TLR4 (rs4986790 and rs4986791) genotypes in 607 Brazilian individuals (208 with chronic gastritis-CG, 174 with gastric cancer-GC and 225 controls -C). RESULTS: The single nucleotide polymorphisms TLR4+1196C/T was not associated with risk of chronic gastritis or gastric cancer and the homozygous genotypes TLR4+896GG and TLR4+1196TT were absent in the studied population. However, the frequency of TLR2 -196 to -174 ins/del + del/del and TLR4+896AG genotypes was significantly higher (P < 0.01 and P = 0.01, respectively) in the cancer group (33.4% and 11.5%, respectively) than in the control group (16.9% and 4.5%, respectively). It was also observed that the G-C haplotype of the TLR4+896A/G+1196C/T (P = 0.02) and the combination of variant alleles of the TLR2/TLR4+896G (P = 0.02) are associated with susceptibility to gastric cancer. In addition, the multiple logistic regression showed that male gender {[}odds ratio (OR) = 2.70; 95% CI: 1.66-4.41; P < 0.01], alcohol intake (OR = 2.93; 95% CI: 1.76-4.87; P < 0.01), TLR2 -196 to -174 del (OR = 2.64; 95% CI: 1.56-4.44; P < 0.01) and TLR4+896G (OR = 3.19; 95% CI: 1.34-7.61; P < 0.01) polymorphisms were associated with a higher susceptibility to developing this neoplasm. CONCLUSION: Our data indicate that TLR2 -196 to -174 del and TLR4+896G may increase the risk of gastric cancer in a Brazilian population. (C) 2012 Baishideng. All rights reserved. (AU)

FAPESP's process: 10/00507-0 - Evaluation of polymorphisms of genes involved in the inflammatory process induced by Helicobacter pylori in the stomach carcinogenesis
Grantee:Ana Elizabete Silva
Support Opportunities: Regular Research Grants