| Full text | |
| Author(s): |
Mercier Franco, Luis Fernando
[1]
;
Pinto de Oliveira, Cristiano Luis
[2]
;
Pessoa Filho, Pedro de Alcantara
[3]
Total Authors: 3
|
| Affiliation: | [1] Univ Sao Paulo, Sch Engn, Chem Engn Grad Program, BR-05424970 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Phys, Dept Expt Phys, BR-05314970 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Sch Engn, Dept Chem Engn, BR-05424970 Sao Paulo, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | AICHE JOURNAL; v. 61, n. 9, p. 2871-2880, SEP 2015. |
| Web of Science Citations: | 1 |
| Abstract | |
An analytical expression for the structure factor for globular proteins in aqueous solution is presented. This expression was obtained considering a potential given by the sum of a hard core, a van der Waals attractive, and a screened Coulomb contribution. Experimental data of small angle x-ray scattering for bovine serum albumin (BSA) in aqueous solutions containing sodium salts at different protein concentrations and pH values are also presented. The developed expression for the structure factor describes accurately these experimental data provided a dependence of the attractive parameter on protein concentration is established. An expression for the osmotic pressure was derived from the structure factor. With attractive parameters adjusted from x-ray scattering data, the osmotic pressure of BSA aqueous solutions could be predicted with very good agreement with experimental data. (c) 2015 American Institute of Chemical Engineers AIChE J, 61: 2871-2880, 2015 (AU) | |
| FAPESP's process: | 11/22070-5 - Thermodynamics of protein solutions: experimental determination and modeling of protein-protein interactions |
| Grantee: | Luís Fernando Mercier Franco |
| Support Opportunities: | Scholarships in Brazil - Doctorate |