Assessing the biochemical changes of tendons of rats in an experimental model of tenotomy under therapeutic ultrasound and LEDs (625 and 945 nm) by near-infrared Raman spectroscopy

Author(s)

Moura Junior, Manoel de Jesus ^[1] ; Martins Maia Filho, Antonio Luis ^[1] ; Pessoa, Diego Rodrigues ^[1] ; Rodrigues Alves, Marcos Danilo ^[1] ; Justino, Jefferson de Sousa ^[1] ; Andrade, Matheus dos Santos ^[1] ; Goncalves Rebelo, Ana Maria ^[1] ; de Lima, Carlos Jose ^[2] ; Barbosa Pinheiro, Antonio Luiz ^{[2, 3]} ; Silveira, Jr., Landulfo ^[2]

Affiliation

^[1] Fac Santo Agostinho, BR-64019625 Teresina, PI - Brazil

^[2] Univ Camilo Castelo Branco UNICASTELO, Inst Biomed Engn, BR-12247016 Sao Jose Dos Campos, SP - Brazil

^[3] Fed Univ Bahia UFBA, Sch Dent, Ctr Biophoton, BR-40110150 Salvador, BA - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 30, n. 6, p. 1729-1738, AUG 2015.
• Web of Science Citations: 1

Abstract

Given the complexity of the tendon repair process, where the neoformed scar tissue tends to prevent or hamper the tendon from performing its normal functions, ultrasound (US) and light-emitting diode (LED) devices have been applied to modulate the tendon repair process. This study aimed to compare the anti-inflammatory and healing effects of therapeutic US and LED in an experimental model of tenotomy using histomorphometry and Raman spectroscopy. Forty Wistar rats (235 +/- 8.3 g) were separated into four groups: tenotomy (TC; control), tenotomy associated with ultrasound (US; 1 MHz, 0.5 W/cm(2)), tenotomy associated with LED at 625 nm (LR; 4 J/cm(2)), and tenotomy associated with LED at 945 nm (LIR; 4 J/cm(2)), in two experimental periods of 7 and 14 days. Histomorphometry showed a decrease of inflammatory cells (macrophages and polymorphonuclear leukocytes) for the group treated with US compared to the TC group (ANOVA, p < 0.0001) and the LIR group compared to the TC group (p < 0.05) at the seventh day, indicating an anti-inflammatory effect. In relation to the LR and TC groups, there was no significant difference. Raman spectroscopy revealed that the LR group presented an increased synthesis of collagen I compared to the other treatments and the TC groups on the 14th day (p < 0.01). US therapy showed an adjuvant effect with anti-inflammatory action, and the LED therapy at 625 nm showed an increase in collagen synthesis, contributing to the process of tendon healing. (AU)

• FAPESP's process: 09/01788-5 - Dispersive Raman spectroscopy using optical fibers "Raman probe" applied to the diagnosis of neoplasia in the skin and prostate
• Grantee: Landulfo Silveira Junior
• Support Opportunities: Regular Research Grants