Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Role of Smoking and Type 2 Diabetes in the Immunobalance of Advanced Chronic Periodontitis

Full text
Author(s):
Duarte, Poliana M. [1] ; Santos, Vanessa R. [1] ; dos Santos, Fernanda A. [1] ; de Lima Pereira, Sanivia A. [2] ; Rodrigues, Denise B. R. [2] ; Napimoga, Marcelo H. [2]
Total Authors: 6
Affiliation:
[1] Univ Guarulhos, Dept Periodont, Dent Res Div, Sao Paulo - Brazil
[2] Univ Uberaba, Lab Biopathol & Mol Biol, Uberaba, MG - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Periodontology; v. 82, n. 3, p. 429-438, MAR 2011.
Web of Science Citations: 16
Abstract

Background: This study evaluates the tissue levels of interleukin (IL)-17(+), IL-15(+), Foxp3(+) cells, fibrosis, and plasma B-cell infiltration in sites with chronic periodontitis in smokers and subjects with type 2 diabetes. Methods: Gingival biopsies were harvested from the following groups: systemically and periodontally healthy subjects (healthy group, n = 10); non-smokers and subjects with advanced periodontitis and without diabetes (non-risk factor/periodontitis group, n = 10); heavy smokers with advanced periodontitis and without diabetes (smoking/periodontitis group, cigarettes per day for at least the past 5 years, n = 10); and non-smoking poorly controlled subjects with diabetes (glycated hemoglobin levels >= 9%) with advanced periodontitis (diabetes mellitus/periodontitis group {[}DMPI, n = 10). The number of IL-17(+), IL-15(+), and Foxp3(+) cells was analyzed by immunohistochemistry, whereas the amount of fibrosis and plasma B-cell infiltration in gingival tissue was analyzed by histomorphometry. Results: The number of Foxp3(+) cells was significantly higher in the periodontitis groups compared to the healthy group (P<0.05). The DMP group presented higher levels of Foxp3(+) cells than other periodontitis groups (P<0.05). The levels of IL-15(+) and IL-17(+) cells and the amount of fibrosis were higher in the DMP group than in the other groups (P<0.05). There was a trend for a decreased B-cell infiltration in the DMP group (P>0.05). There was a slightly significant negative correlation between B-cell infiltration and the amount of fibrosis (P<0.05). Conclusion: Upregulation of IL-17(+), IL-15(+), and Foxp3(+) cells and increased amounts of fibrosis were observed in chronic periodontitis sites in subjects with type 2 diabetes, suggesting that periodontitis development in these subjects may be influenced by the T helper 17/T regulatory axis. J Periodontol 2011;82:429-438. (AU)

FAPESP's process: 08/09687-0 - Periodontal treatment in controlled and uncontrolled Diabetic subjects: clinical, glycemic and cytokine and bone resorption related factors evaluations
Grantee:Poliana Mendes Duarte
Support type: Regular Research Grants