| Full text | |
| Author(s): |
Total Authors: 3
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| Affiliation: | [1] Fundacao Univ Fed Grande Dourados, BR-79804970 Dourados, MS - Brazil
[2] Univ Fed Lavras, Lavras, MG - Brazil
[3] Univ Fed Vicosa, Vicosa, MG - Brazil
Total Affiliations: 3
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| Document type: | Journal article |
| Source: | ACTA SCIENTIARUM-AGRONOMY; v. 37, n. 3, p. 313-320, JUL-SEP 2015. |
| Web of Science Citations: | 5 |
| Abstract | |
This research aimed to investigate the cellular alterations during the loss and re-establishment of desiccation tolerance (DT) in germinated Sesbania virgata seeds. The loss of DT was characterized in germinated seeds with increasing radicle lengths (1, 2, 3, 4 and 5 mm) when subjected to dehydration in silica gel, followed by rehydration. To re-establish DT, the germinated seeds were incubated for 72h in polyethylene glycol (PEG, -2.04 MPa) with or without ABA (100 mu M) before dehydration in silica gel. Cell viability was assessed by seedling survival, and DNA integrity was evaluated by gel electrophoresis. Seeds with 1 mm radicle length survived dehydration to the original moisture content (MC) of the dry seed (approximately 10%). PEG treatment was able to re-establish DT, at least partially, with 2, 3 and 4 mm but not in 5 mm radicle lengths. Germinated seeds treated with PEG+ABA performed better than those treated only with PEG, and DT was re-established even in germinated seeds with a 5 mm radicle length. Among the PEG-treated germinated seeds dehydrated to 10% MC, DNA integrity was maintained only in those with a 1 mm radicle length. (AU) | |
| FAPESP's process: | 05/04139-7 - Carbohydrates of tropical species as modulators of ecophysiological processes and as environmental stress response markers |
| Grantee: | Marcia Regina Braga |
| Support Opportunities: | Research Projects - Thematic Grants |