Association of Nitric Oxide Synthase and Matrix Metalloprotease Single Nucleotide Polymorphisms with Preeclampsia and Its Complications

Author(s):Show less -

Leonardo, Daniela P. ^[1] ; Albuquerque, Dulcinela M. ^[1] ; Lanaro, Carolina ^[1] ; Baptista, Leticia C. ^[1] ; Cecatti, Jose G. ^[2] ; Surita, Fernanda G. ^[2] ; Parpinelli, Mary A. ^[2] ; Costa, Fernando F. ^[1] ; Franco-Penteado, Carla F. ^[1] ; Fertrin, Kleber Y. ^{[1, 3]} ; Costa, Maria Laura ^[2]

Affiliation

^[1] Univ Campinas UNICAMP, Hematol & Hemotherapy Ctr, Sao Paulo - Brazil

^[2] Univ Campinas UNICAMP, Sch Med, Dept Obstet & Gynaecol, Sao Paulo - Brazil

^[3] Univ Campinas UNICAMP, Sch Med, Dept Clin Pathol, Sao Paulo - Brazil

• Document type: Journal article
• Source: PLoS One; v. 10, n. 8 AUG 28 2015.
• Web of Science Citations: 13

Abstract

Background Preeclampsia is one of the leading causes of maternal and neonatal morbidity and mortality in the world, but its appearance is still unpredictable and its pathophysiology has not been entirely elucidated. Genetic studies have associated single nucleotide polymorphisms in genes encoding nitric oxide synthase and matrix metalloproteases with preeclampsia, but the results are largely inconclusive across different populations. Objectives To investigate the association of single nucleotide polymorphisms (SNPs) in NOS3 (G894T, T-786C, and a variable number of tandem repetitions VNTR in intron 4), MMP2 (C-1306T), and MMP9 (C-1562T) genes with preeclampsia in patients from Southeastern Brazil. Methods This prospective case-control study enrolled 77 women with preeclampsia and 266 control pregnant women. Clinical data were collected to assess risk factors and the presence of severe complications, such as eclampsia and HELLP (hemolysis, elevated liver enzymes, and low platelets) syndrome. Results We found a significant association between the single nucleotide polymorphism NOS3 T-786C and preeclampsia, independently from age, height, weight, or the other SNPs studied, and no association was found with the other polymorphisms. Age and history of preeclampsia were also identified as risk factors. The presence of at least one polymorphic allele for NOS3 T-786C was also associated with the occurrence of eclampsia or HELLP syndrome among preeclamptic women. Conclusions Our data support that the NOS3 T-786C SNP is associated with preeclampsia and the severity of its complications. (AU)

• FAPESP's process: 14/00984-3 - Red blood cell disorders: pathophysiology and new therapeutic approaches
• Grantee: Fernando Ferreira Costa
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 08/57441-0 - Clinical, cellular and molecular alterations in hemoglobinopathies and other hereditary hemolytic anemias
• Grantee: Fernando Ferreira Costa
• Support Opportunities: Research Projects - Thematic Grants