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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Participation of TNF-alpha in Inhibitory Effects of Adipocytes on Osteoblast Differentiation

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Author(s):
Abuna, Robrigo P. F. [1] ; De Oliveira, Fabiola S. [1] ; Santos, Thiago De S. [1] ; Guerra, Thais R. [1] ; Rosa, Adalberto L. [1] ; Beloti, Marcio M. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Cell Culture Lab, BR-14040904 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Cellular Physiology; v. 231, n. 1, p. 204-214, JAN 2016.
Web of Science Citations: 9
Abstract

Mesenchymal stem cells from bone marrow (BM-MSCs) and adipose tissue (AT-MSCs) are attractive tools for cell-based therapies to repair bone tissue. In this study, we investigated the osteogenic and adipogenic potential of BM-MSCs and AT-MSCs as well as the effect of crosstalk between osteoblasts and adipocytes on cell phenotype expression. Rat BM-MSCs and AT-MSCs were cultured either in growth, osteogenic, or adipogenic medium to evaluate osteoblast and adipocyte differentiation. Additionally, osteoblasts and adipocytes were indirectly co-cultured to investigate the effect of adipocytes on osteoblast differentiation and vice versa. BM-MSCs and AT-MSCs exhibit osteogenic and adipogenic potential under non-differentiation-inducing conditions. When exposed to osteogenic medium, BM-MSCs exhibited higher expression of bone markers compared with AT-MSCs. Conversely, under adipogenic conditions, AT-MSCs displayed higher expression of adipose tissue markers compared with BM-MSCs. The presence of adipocytes as indirect co-culture repressed the expression of the osteoblast phenotype, whereas osteoblasts did not exert remarkable effect on adipocytes. The inhibitory effect of adipocytes on osteoblasts was due to the release of tumor necrosis factor alpha (TNF-) in culture medium by adipocytes. Indeed, the addition of exogenous TNF- in culture medium repressed the differentiation of BM-MSCs into osteoblasts mimicking the indirect co-culture effect. In conclusion, our study showed that BM-MSCs are more osteogenic while AT-MSCs are more adipogenic. Additionally, we demonstrated the key role of TNF- secreted by adipocytes on the inhibition of osteoblast differentiation. Thus, we postulate that the higher osteogenic potential of BM-MSCs makes them the first choice for inducing bone repair in cell-based therapies. J. Cell. Physiol. 230: 204-214, 2016. (c) 2015 Wiley Periodicals, Inc. (AU)

FAPESP's process: 11/10658-8 - Effect of the poly(vinylidene-trifluoroethylene)/barium titanate membrane on the bone formation in rat cavariae defects
Grantee:Márcio Mateus Beloti
Support Opportunities: Regular Research Grants