| Full text | |
| Author(s): |
de Oliveira, Cristina Mendes
[1, 2]
;
Bravo, Ignacio G.
[2]
;
Santiago e Souza, Nathalia Caroline
[1]
;
Nogueira Dias Genta, Maria Luiza
[3]
;
Tavares Guerreiro Fregnani, Jose Humberto
[4]
;
Tacla, Maricy
[5]
;
Carvalho, Jesus Paula
[3]
;
Longatto-Filho, Adhemar
[6, 7, 8, 9]
;
Levi, Jose Eduardo
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Inst Med Trop, Lab Virol, Sao Paulo - Brazil
[2] Catalan Inst Oncol, Infect & Canc Lab, Barcelona - Spain
[3] ICESP, Fac Med, Sao Paulo - Brazil
[4] Barretos Canc Hosp, Teaching & Res Inst, Sao Paulo - Brazil
[5] Univ Sao Paulo, Fac Med, Dept Gynecol, Sao Paulo - Brazil
[6] Univ Minho, Sch Hlth Sci, Life & Hlth Sci Res Inst ICVS, Braga - Portugal
[7] Univ Sao Paulo, Fac Med, Lab Med Invest LIM 14, Sao Paulo - Brazil
[8] Hosp Canc Barretos, Mol Oncol Res Ctr, Sao Paulo - Brazil
[9] PT Govt Associate Lab, ICVS 3Bs, Braga - Portugal
Total Affiliations: 9
|
| Document type: | Journal article |
| Source: | INFECTION GENETICS AND EVOLUTION; v. 34, p. 44-51, AUG 2015. |
| Web of Science Citations: | 10 |
| Abstract | |
Invasive cervical cancer (ICC) is the third most frequent cancer among women worldwide and is associated with persistent infection by carcinogenic human papillomaviruses (HPVs). The combination of large populations of viral progeny and decades of sustained infection may allow for the generation of intra-patient diversity, in spite of the assumedly low mutation rates of PVs. While the natural history of chronic HPVs infections has been comprehensively described, within-host viral diversity remains largely unexplored. In this study we have applied next generation sequencing to the analysis of intra-host genetic diversity in ten ICC and one condyloma cases associated to single HPV16 infection. We retrieved from all cases near full-length genomic sequences. All samples analyzed contained polymorphic sites, ranging from 3 to 125 polymorphic positions per genome, and the median probability of a viral genome picked at random to be identical to the consensus sequence in the lesion was only 40%. We have also identified two independent putative duplication events in two samples, spanning the L2 and the L1 gene, respectively. Finally, we have identified with good support a chimera of human and viral DNA. We propose that viral diversity generated during HPVs chronic infection may be fueled by innate and adaptive immune pressures. Further research will be needed to understand the dynamics of viral DNA variability, differentially in benign and malignant lesions, as well as in tissues with differential intensity of immune surveillance. Finally, the impact of intralesion viral diversity on the long-term oncogenic potential may deserve closer attention. (C) 2015 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 11/24035-2 - Whole genome comparison of HPV-11 and 16 isolates involved in asymptomatic infection, benign and malignant lesions |
| Grantee: | Cristina Mendes de Oliveira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 12/23290-1 - Whole genome comparison of HPV 11 and 16 isolates involved in asymptomatic infection, benign and malignant lesions |
| Grantee: | Cristina Mendes de Oliveira |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |