Whole genome comparison of HPV-11 and 16 isolates involved in asymptomatic infection, benign and malignant lesions

• Grant number: 11/24035-2
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2012
• End date: March 31, 2015
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: José Eduardo Levi
• Grantee: Cristina Mendes de Oliveira
• Host Institution: Instituto de Medicina Tropical de São Paulo (IMT). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 12/23290-1 - Whole genome comparison of HPV 11 and 16 isolates involved in asymptomatic infection, benign and malignant lesions, BE.EP.PD

Abstract

Human Papillomavirus (HPV) can cause benign lesions such as skin warts, condylomata, epidermodysplasia; and malignant lesions, especially invasive cervical cancer. According to the World Health Organization estimators, there are more than 500,000 new cases of invasive cervical cancer annually and more than 250,000 deaths, of which 80% occur in developing countries. HPV types that infect the anogenital region can be classified according to their oncogenic potential into: high-risk and low-risk HPV types. There are descriptions of high-risk HPV types associated with benign lesions, as well as the opposite is true, ie, low-risk HPV types associated to malignant lesions. Until this moment, it is not clear why viruses belonging to the same HPV type cause no or very distinct proliferative responses. Molecular studies have identified HPV variants, confering different risks of progression to invasive cervical cancer, using the URR region. Another study was able to associate the E5 protein to the phenotype. These findings suggest that a single HPV region is not enough to determine the phenotype. However, no study so far has evaluated, in this context, full length viruses. The present study aims to sequence and analyze the complete genome of HPV-16, prototype of high-risk HPVs, and HPV-11, prototype of low-risk HPVs from samples displaying normal cytology, benign and malignant lesions, aiming to identify molecular patterns correlating to the rare phenotypes.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, CRISTINA MENDES; BRAVO, IGNACIO G.; SANTIAGO E SOUZA, NATHALIA CAROLINE; NOGUEIRA DIAS GENTA, MARIA LUIZA; TAVARES GUERREIRO FREGNANI, JOSE HUMBERTO; TACLA, MARICY; CARVALHO, JESUS PAULA; LONGATTO-FILHO, ADHEMAR; LEVI, JOSE EDUARDO. High-level of viral genomic diversity in cervical cancers: A Brazilian study on human papillomavirus type 16. INFECTION GENETICS AND EVOLUTION, v. 34, p. 44-51, AUG 2015. (11/24035-2, 12/23290-1)

PIMENOFF, VILLE N.; MENDES DE OLIVEIRA, CRISTINA; BRAVO, IGNACIO G.. Transmission between Archaic and Modern Human Ancestors during the Evolution of the Oncogenic Human Papillomavirus 16. Molecular Biology and Evolution, v. 34, n. 1, p. 4-19, JAN 2017. (11/24035-2, 12/23290-1)